Taxonomy of Celtis (Ulmaceae) in Taiwan Based on Leaf Morphologies, ITS and ISSR Molecular Markers

• Main Authors: Chi-Feng Chang, 張淇鋒
• Other Authors: Kuen-Yih Ho
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/5f2yme

碩士 === 國立嘉義大學 === 森林暨自然資源研究所 === 97 === 【Abstract】 Hackberry plant (Celtis L.) is subordinated to Ulmaceae. The identification of these species are difficult because of the similarity of leaf appearance. This study examined the morphological characters of leaves, and both ITS (internal transcribed spacer in rDNA) and ISSR (inter-simple sequence repeat) molecular markers to differentiate the species in Celtis. From 27 nature sites in Taiwan totally 192 samples were collected, and 13 leaf characteristics were measured, then via UPGMA to build the phylogenetic tree. The results showed that Celtis nervosa, C. biondii and C. philippensis were distinctly classified into three groups, and closer relationship existed between C. sinensis and C. formosana. In ITS experiment, cloning and amplifying the 5.8 rDNA, then using MP and NJ to proceed series analysis and affinity pedigree were built. It’s also concluded that the taxonomy of C. sinensis and C. formosana were more closer. Otherwise, using ISSR to analysis genetic variation of C. sinensis and C. formosana, testing ISSR primers by PCR, and there were 9 pairs of primers selected in genetic analysis. After all, the genetic distance between species were calculated, and the dendrogram were erected by UPGMA. The results showed that among the 71 bands, 51 were polymorphic (71.8 %). The POPGENE showed that Gst of C. sinensis and C. formosana up was to 0.3814, and the Nm was 0.8110, revealed that the obvious differentiation between C. sinensis and C. formosana. The AMOVA refered to interspecific exchange were 57.38 % (p <0.0001), and the intraspecific exchange were 42.62 % (p＜0.0001), it showed that the probability of gene exchange were low, the interspecific exchange were the main factor for variation. Mantel test was used to examine the relationship of elevation and heredity between C. sinensis and C. formosana, the result showed highly significant (r = 0.33841，P = 0.0000), and the genetic variation connected to elevation. It is possible that the elevation difference brought about ecotype and differentiation, then accelerated the speciation. Based on these results, that the hackberry plants in Taiwan could be divide into five species, C. sinensis, C. formosana, C. nervosa, C. biondii and C. philippensis.