The Study of Automatic Tracking and Manipulation for Cell Lysis Using Machine Vision
碩士 === 國立嘉義大學 === 生物機電工程學系研究所 === 97 === An automatic platform for cell localization using image processing and electroosmotic flow technology was developed and used the in vivo human leukemic cells (U937) experiments. The in vivo cells were located successfully in the assigned area by aluminum...
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ndltd-TW-097NCYU57300022019-05-15T19:49:42Z http://ndltd.ncl.edu.tw/handle/q6d4r5 The Study of Automatic Tracking and Manipulation for Cell Lysis Using Machine Vision 利用機器視覺自動追蹤與操控細胞裂解之研究 Chih-Sheng Hsu 許智昇 碩士 國立嘉義大學 生物機電工程學系研究所 97 An automatic platform for cell localization using image processing and electroosmotic flow technology was developed and used the in vivo human leukemic cells (U937) experiments. The in vivo cells were located successfully in the assigned area by aluminum electrode with lithography process. The cells were lysised with electroporation then DNA was collected in this study. First, a CCD was used to take video from microscope and then a PCI image card acquired the image data to computer. The program was designed to find cell location and trace the in vivo cell and then the cell driving mechanism is started through the voltage or time of electrode controlled with fuzzy logic method by LabVIEW package software. In addition, the XY platform is automatically controlled to keep the cell within the field of view. When the in vivo cell enters the assigned lysis area, the cell will be electroporated by the electrode. The monitoring software was developed to track and manipulate single in vivo cell to lysis successfully in this study. It can control cell move to the assigned location fatly. Chyung Ay Min-Sheng Hung 艾群 洪敏勝 學位論文 ; thesis zh-TW |
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碩士 === 國立嘉義大學 === 生物機電工程學系研究所 === 97 === An automatic platform for cell localization using image processing and electroosmotic flow technology was developed and used the in vivo human leukemic cells (U937) experiments. The in vivo cells were located successfully in the assigned area by aluminum electrode with lithography process. The cells were lysised with electroporation then DNA was collected in this study.
First, a CCD was used to take video from microscope and then a PCI image card acquired the image data to computer. The program was designed to find cell location and trace the in vivo cell and then the cell driving mechanism is started through the voltage or time of electrode controlled with fuzzy logic method by LabVIEW package software. In addition, the XY platform is automatically controlled to keep the cell within the field of view. When the in vivo cell enters the assigned lysis area, the cell will be electroporated by the electrode.
The monitoring software was developed to track and manipulate single in vivo cell to lysis successfully in this study. It can control cell move to the assigned location fatly.
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Chyung Ay |
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Chyung Ay Chih-Sheng Hsu 許智昇 |
author |
Chih-Sheng Hsu 許智昇 |
spellingShingle |
Chih-Sheng Hsu 許智昇 The Study of Automatic Tracking and Manipulation for Cell Lysis Using Machine Vision |
author_sort |
Chih-Sheng Hsu |
title |
The Study of Automatic Tracking and Manipulation for Cell Lysis Using Machine Vision |
title_short |
The Study of Automatic Tracking and Manipulation for Cell Lysis Using Machine Vision |
title_full |
The Study of Automatic Tracking and Manipulation for Cell Lysis Using Machine Vision |
title_fullStr |
The Study of Automatic Tracking and Manipulation for Cell Lysis Using Machine Vision |
title_full_unstemmed |
The Study of Automatic Tracking and Manipulation for Cell Lysis Using Machine Vision |
title_sort |
study of automatic tracking and manipulation for cell lysis using machine vision |
url |
http://ndltd.ncl.edu.tw/handle/q6d4r5 |
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