The Study of Automatic Tracking and Manipulation for Cell Lysis Using Machine Vision

• Main Authors: Chih-Sheng Hsu, 許智昇
• Other Authors: Chyung Ay
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/q6d4r5

碩士 === 國立嘉義大學 === 生物機電工程學系研究所 === 97 === An automatic platform for cell localization using image processing and electroosmotic flow technology was developed and used the in vivo human leukemic cells (U937) experiments. The in vivo cells were located successfully in the assigned area by aluminum electrode with lithography process. The cells were lysised with electroporation then DNA was collected in this study. First, a CCD was used to take video from microscope and then a PCI image card acquired the image data to computer. The program was designed to find cell location and trace the in vivo cell and then the cell driving mechanism is started through the voltage or time of electrode controlled with fuzzy logic method by LabVIEW package software. In addition, the XY platform is automatically controlled to keep the cell within the field of view. When the in vivo cell enters the assigned lysis area, the cell will be electroporated by the electrode. The monitoring software was developed to track and manipulate single in vivo cell to lysis successfully in this study. It can control cell move to the assigned location fatly.