| Summary: | 碩士 === 國立東華大學 === 生物技術研究所 === 97 === Pyrethroids are the most wide-used pesticide in the world because they are highly toxic to aquatic creatures and may have endocrine disruption activity to mammals. The major detection techniques for pyrethroids are HPLC and GC. Quartz Crystal Microbalance (QCM) is a detection platform base on the frequency change as chemicals attached to quartz resonator. Molecularly imprinted polymer (MIP) is a sensing material building method that uses target or agonist as a template and small monomer polymerized to form target-specific polymer. This study was to build a liquid-phase pyrethroid detection system using MIP and QCM. 2-propene-1-thiol was applied for surface modifiers, and 3-phenoxyphenylactonitrile as a template, and N-benzylacrylamide, acrylamide, and acrylic acid chose as monomers to make polymer to examine cypermethrin as a target. The result showed that surface modification was not necessary in this system. The existence of DMSO decreased the frequency change of cypermethrin. The best monomer: template ratio was found to frequency change of cypermethrin. Concentration of monomer solution was not related to frequency change of cypermethrin. The detection limit of the system was 1 μg / L of cypermethrin and frequency change reached a plateau in 10 μg / L. Using atrazine and difenoconazol as interferers of cypermethrin in this system showed that the system had not interfered with those chemicals. This study implies that QCM and MIP for building pyrethroid detection system can detect cypermethrin at 1 μg / L with no significant interference.
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