In Vitro Study of Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells on Different Implant Surfaces

• Main Authors: Ming-Hsuan Wen, 温明璇
• Other Authors: Chung Shih
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/68937278836122915973

碩士 === 國防醫學院 === 生物及解剖學研究所 === 97 === Human bone marrow mesenchymal stem cells (hBMSCs) are multipotential cells which can differentiate into osteogenic and other cell lineages. Titanium (Ti) implants are common metal implant widely used in orthopedic and oral maxillofacial reconstructive surgery. Ti implant directly bond to bone depended on chemical and physical properties of the surface. However, the bonding and bone ingrowth between pure Ti implant and bone are slow process and incomplete osseointegration. Ti implant with specific surface modifications which increase surface roughness or mimic bioactive materials can improve the cell adhesion, proliferation and differentiation. Nevertheless, previous studies have been focused on integration between fibroblasts, osteoblasts or bone marrow stromal cells and implant surface. Till now, interrelationship/interaction between hBMSCs or marrow stromal cells and different surface modification of implant are still unclear. Therefore, we investigated the changes in osteogenic potential of hBMSCs on different modified surfaces of Ti implant using scanning electron microscopy, bromodeoxyuridine (BrdU) assay, alkaline phosphatase (ALP) activity, and reverse transcriptase polymerase chain reaction (RT-PCR). Compared to the pure Ti, hBMSCs adhesion was changed due to roughness produced by plasma spraying. With BrdU assay, there was no significant difference on cell proliferation between three modified surfaces. The results of ALP activity and RT-PCR indicated that the osteogenic potential of hBMSC was lower on the Ti plasma spraying and plasma sprayed hydroxyapatite (HA) coating surfaces than on polished Ti surface. The results of this study demonstrated that roughness and HA coating produced by plasma spraying interfered with osteogenic differentiation of hBMSCs.