| Summary: | 碩士 === 南華大學 === 自然醫學研究所 === 97 === This research utilized far infrared radiation (FIR) emitted by far infrared material (FIM) with different wavelength to irradiate peripheral blood monocytes, bone marrow stem cells and rats over different time spans for investigating the effect of FIR on immunoregulation.
In the experiments, the wavelength of FIM was set as 9.63 μm, 9.11 μm or 8.57 μm, and the irradiation time was 15, 30 or 45 min. In cell tests, the proliferation rate of macrophages was determined by MTT assay, and the activity of macrophages was determined by TRAP staining. The cultured medium was collected for TNF-αand IL-1β analysis. RNA of the peripheral blood monocytes was extracted to analyze the gene expression of IL-6 and IL-8. Moreover, bone marrow cells were cultured in vivo to investigate the differentiation of stromal cells, lipocytes as well as osteoblasts and to evaluate the development of microenvironment in bone marrow. In animal experiments, theλmax was set as 9.63 μm or 9.11 μm, and the irradiation time was 45 min for every test. Two irradiation modes were utilized in the tests. The first is continuous irradiation for 2 weeks. Concentration of TNF-α and IL-1β in the serum of rats were determined respectively on the 3 rd, 7 th and 14 th day. The second is once irradiation, and the concentration of TNF-α and IL-1β in the serum was assayed at the 2 nd and 4 th hour after irradiation.
The results indicate that FIR is able to enhance proliferation and development of macrophages and increase secretion of IL-6, IL-8 and TNF-α. Development of microenvironment in bone marrow can also be promoted after FIR irradiation, which can further stimulate differentiation of hematopoietic stem cells. Additionally, IL-6 in serum of the rats increased at the 2 nd and 4 th hour after FIR irradiation. The secretion of IL-6 and IL-8 in serum was also enhanced after 2 weeks of irradiation. Accordingly, FIR is suggested capable of promoting immunity of organisms by increasing secretion of cytokines (IL-6, IL-8 and TNF-α), enhancing development of bone marrow microenvironment as well as the differentiation of hematopoietic stem cells. This can further promote development and differentiation of the macrophages and thus regulation immunity of organisms.
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