Adjuvanicity of Porcine IL-18 on Japanese Encephalitis Virus (JEV) DNA Vaccine

• Main Authors: Yu-Hsiang Hsieh, 謝宇翔
• Other Authors: Hso-Chi Chaung
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/69519272974577743162

碩士 === 國立屏東科技大學 === 生物科技研究所 === 97 === Interleukin-18 (IL-18) can enhance the amounts of IFN-γ secreted by T cells and decreases the production of IL-10, and thus reinforces the adpative immunity reaction. This experiment uses Chinese hamster ovary cells (CHO cells) to analyze the transfection efficiency of eukaryotic expression of porcine IL-18 plasmid, and uses the 4 weeks old specific-pathogen free (SPF) pigs to test it and take it as the adjuvant benefit of Japanese encephalitis virus (JEV) DNA vaccine. The result demonstrates that the growing amounts of the splenocytes IL-12 and IFN-γ from the animals injected with IL-18 adjuvant are higher than the pigs that are only injected with JEV DNA vaccine. Moreover, the gene expression of IFN-α and TLR3, 7 and 8 increase yield in the animals injected with IL-18 adjuvant. Therefore, eukaryotic expression of porcine IL-18 plasmid owns the ability to adjust the immune effect of the JEV DNA vaccine that is injected at the same time, which can effectively improve the function of the vaccine.