Summary: | 碩士 === 國立屏東科技大學 === 植物保護系所 === 97 === Abstract
Student ID: M9415002
Title of Thesis: Study on the cultivation of Hericium erinaceus
( Bull. ex Fr. ) Pers. for promoting mushroom yield
Total Page: 129
Name of Institute : Department of Plant Protection, National Pingtung
University of Science and Technology.
Graduate Date:July, 2009 Degree Conferred: Master
Name of Student: Hsiu-Cheng Lu Adviser Professor: Wen-Jinn Liang
The Contents of Abstract in This Thesis:
Hericium erinaceus (Bull. ex Fr.) Pers. is a traditional edible and medical mushroom in China, with common names such as Lion’s Mane, Monkey’s Head or Houtou mushroom. Due to the demand of fresh mushroom of H. erinaceus for human health care in recent years in Taiwan, the cultivation of Houtou mushroom increases year after year. Most of the cultivation has been conducted under non-air-conditioned culture room, and limits the harvest period from early winter to end of spring. Therefore yield and quality of Houtou mushroom are varied depending on climate and culture technology. This research was focused on cultural characteristic of H. erinaceus, recycling of used waste sawdust for decreasing the sawdust supply and promoting the cultivation technology of nutrient supplement and culture management for cultivation of Houtou mushroom. On PDA medium, H. erinaceus mycelia grow well at 24~28℃ of temperature and at pH 5~6. In screening of suitable materials for spawn, sawdust and Job’s tear are better than wheat grain in mushroom production. The yields of mushroom of first flush between 5 isolates of H. erinaceus cultivated on sawdust with bag culture are not different significantly. The mushroom yields of H. erinaceus are also not significantly different between PP plastic bag culture and bottle culture. Comparing the sawdust culture substrates of hardwood (of Acacia confusa), and soft wood ( of Thuja plicata), and the mixtures of various proportions of hardwood and soft wood, the mixture sawdust of 50% both hardwood and soft wood, for mushroom yield of H. erinaceus from three flushes in the biological efficiency(BE), is the best with more than 70% when cultivated at 18~20℃ is the effect of on stimulation of mushroom development, the whole fullness of pine sawdust substrate was found best 68% with BE (biological efficiency) from two flushes’ yield of Houtou mushroom that cultivated at 18~20℃. Comparing composted new sawdust to the recycled ones, the BE of first flush yield of mushroom in all sawdust substrates are not different significantly. In the recycled waste sawdust for cultivation the Houtou mushroom, all of the recycled sawdust got the BE of first yield, higher than those of new sawdust, one even reached 33% of BE. Comparing the composted recycled sawdust to the noncomposted, the noncomposted one has 34% BE, which was 40% higher than the composted new sawdust materials. Comparison of the sawdust recycled from different mushroom production, among the Maitake mushroom (Grifola fzondosa), Nameko mushroom (Pholita nameko), King Oyster (Pleurotus erynqii), Himematsutake mushroom (Aqaricus blazei) and Houtou mushroom, the sawdust containing 50% King Oyster waste sawdust had 33% BE, that was 40% better than the new one, and the results also showed that sawdust used by Houtou mushroom production were the better recycle for cultivating Houtou mushroom than those used by other mushrooms, with 20% ~ 40% increase in BE, respectively. In nutrient supplement, 6%、8% or 10% rice bran added to pine sawdust culture substrates all increased the yields by 20% or more. Comparing the effects of corn powder rice bran and wheat bran supplemented in different contents of pine sawdust on mycelial growth of Houtou mushroom, the results showed that corn powder supplements were superior to other nutrient supplements, and the more content of corn powder added the better the mycelia grow. In the effect of supplemented to pine sawdust culture substrate on mushroom yield, 3% Pangolagrass chicken manure compost was higher than that of 1%, 5% supplements or no supplement effects of pH valued and water contents of pine sawdust culture substrates on mycelial growth in glass tube and yield of mushroom in bottle culture, the Houtou mushroom mycelia grew well at pH 5.8~6.4 and fruiting body developed at pH 5.8 and 5.9 with 39.8% and 42.5% of BE, respectively Houtou mushroom grows its mycelia and develops its fruit bodies better at higher water content of sawdust substrates than at lower ones. It got 51% of BE in first flush production at 72.2% water content. When H. erinaceus was cultivated on pine sawdust culture substrates in PP-bottle, it took 30 ~ 34 days from inoculation to harvesting mushroom in all different pH values or water contents of sawdust culture substrates.
Key wordS: Hericium erinaceus, cultivation, biological efficiency, utilization of mushroom waste culture substrates
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