Use of gene analysis to evaluate the groundwater microbial bioremediation processes of a TCE-contaminated site
碩士 === 國立中山大學 === 生物科學系研究所 === 97 === The industrial solvent trichloroethylene (TCE) is among the most ubiquitous chlorinated compounds found in groundwater pollution. TCE in environment can be removed by physical, chemical and biological procedures. The objective of this pilot-scale study was to ap...
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ndltd-TW-097NSYS51120202019-05-15T19:27:46Z http://ndltd.ncl.edu.tw/handle/j7nfph Use of gene analysis to evaluate the groundwater microbial bioremediation processes of a TCE-contaminated site 以基因分析法評估三氯乙烯污染地下水之微生物整治成效 Wei-chen Liu 劉瑋晨 碩士 國立中山大學 生物科學系研究所 97 The industrial solvent trichloroethylene (TCE) is among the most ubiquitous chlorinated compounds found in groundwater pollution. TCE in environment can be removed by physical, chemical and biological procedures. The objective of this pilot-scale study was to apply an enhanced in situ bioremediation technology to remediate TCE-contaminated groundwater. Both aerobic and anaerobic remedial systems were evaluated at a TCE-spill site located in southern Taiwan. In the aerobic test zone, the effectiveness of air, nutrient, and sugarcane molasses injection to enhance the aerobic cometabolism on TCE degradation was evaluated. In the anaerobic test zone, the effectiveness of nutrient and sugarcane molasses injection to enhance the anaerobic reductive dechlorination on TCE degradation was also evaluated. Polymerase chain reaction was applied to analyze the gene variation in TCE-microbial degraders during the treatment process. Results from this study indicate that the aerobic TCE-degraders (type Ⅱ methanotrophs) and the gene of degradation enzymes (toluene monooxygenase, toluene dioxygenase, particulate methane monooxygenase) were detected after the treatment process in the aerobic test zone. Moreover, TCE concentration dropped from approximately 0.1 mg/L to below 0.05 mg/L in the aerobic test zone after six months of treatment. In the anaerobic treatment zone, Dehalococcoides (anaerobic TCE-degrader) and the gene of degradation enzyme (vcrA) were detected and a significant drop of TCE concentration was also observed. Results reveal that both the aerobic cometabolism and anaerobic dechlorination are feasible and applicable technologies to clean up TCE contaminated aquifers. Jimmy C. M. Kao Jong-Kang Liu 高志明 劉仲康 2009 學位論文 ; thesis 95 zh-TW |
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碩士 === 國立中山大學 === 生物科學系研究所 === 97 === The industrial solvent trichloroethylene (TCE) is among the most ubiquitous chlorinated compounds found in groundwater pollution. TCE in environment can be removed by physical, chemical and biological procedures. The objective of this pilot-scale study was to apply an enhanced in situ bioremediation technology to remediate TCE-contaminated groundwater. Both aerobic and anaerobic remedial systems were evaluated at a TCE-spill site located in southern Taiwan. In the aerobic test zone, the effectiveness of air, nutrient, and sugarcane molasses injection to enhance the aerobic cometabolism on TCE degradation was evaluated. In the anaerobic test zone, the effectiveness of nutrient and sugarcane molasses injection to enhance the anaerobic reductive dechlorination on TCE degradation was also evaluated. Polymerase chain reaction was applied to analyze the gene variation in TCE-microbial degraders during the treatment process. Results from this study indicate that the aerobic TCE-degraders (type Ⅱ methanotrophs) and the gene of degradation enzymes (toluene monooxygenase, toluene dioxygenase, particulate methane monooxygenase) were detected after the treatment process in the aerobic test zone. Moreover, TCE concentration dropped from approximately 0.1 mg/L to below 0.05 mg/L in the aerobic test zone after six months of treatment. In the anaerobic treatment zone, Dehalococcoides (anaerobic TCE-degrader) and the gene of degradation enzyme (vcrA) were detected and a significant drop of TCE concentration was also observed. Results reveal that both the aerobic cometabolism and anaerobic dechlorination are feasible and applicable technologies to clean up TCE contaminated aquifers.
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author2 |
Jimmy C. M. Kao |
author_facet |
Jimmy C. M. Kao Wei-chen Liu 劉瑋晨 |
author |
Wei-chen Liu 劉瑋晨 |
spellingShingle |
Wei-chen Liu 劉瑋晨 Use of gene analysis to evaluate the groundwater microbial bioremediation processes of a TCE-contaminated site |
author_sort |
Wei-chen Liu |
title |
Use of gene analysis to evaluate the groundwater microbial bioremediation processes of a TCE-contaminated site |
title_short |
Use of gene analysis to evaluate the groundwater microbial bioremediation processes of a TCE-contaminated site |
title_full |
Use of gene analysis to evaluate the groundwater microbial bioremediation processes of a TCE-contaminated site |
title_fullStr |
Use of gene analysis to evaluate the groundwater microbial bioremediation processes of a TCE-contaminated site |
title_full_unstemmed |
Use of gene analysis to evaluate the groundwater microbial bioremediation processes of a TCE-contaminated site |
title_sort |
use of gene analysis to evaluate the groundwater microbial bioremediation processes of a tce-contaminated site |
publishDate |
2009 |
url |
http://ndltd.ncl.edu.tw/handle/j7nfph |
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