| Summary: | 碩士 === 國立清華大學 === 分子醫學研究所 === 97 === Cell patterning techniques are the basis to construct in vivo-like complex tissues and organs. Although many cell patterning methods have been developed so far, most of them are either sophisticated or depend on special devices. Here, we present a simple and efficient cell patterning method which is based on magnetic force and photosensitive poly (ethylene glycol) diacrylate (PEG-DA) hydrogels. The PEG-DA hydrogel was made magnetic by mixing with magnetic microparticles and fabricated into specific patterns by photolithography. The resolution of this approach in microstructure fabrication was shown to be about 150 μm. In addition, the patterned PEG hydrogels can be manipulated by using magnetic force and transported to desired locations without direct contact with the magnetic probe. To create cell patterns, the magnetic PEG-DA blocks were placed into a cell culture dish and hold at a designated position with a magnet. Cells were then seeded on the areas not covered by the hydrogel and allowed to attach. After the cells were adhered tightly to the dish surface, the hydrogel was removed, again by magnetic manipulation. The second types of cells were then loaded into the culture dish to grow on the unoccupied areas to form heterotypic cell patterns. Using this method, many complex cell patterns can be generated with good resolutions. This method produces negligible effects on cell viability, thus eliminating potential cell harmful effects associated with other cell manipulation methods. Together, these findings indicate that this simple cell patterning method will be a useful tool in cell biology and tissue engineering.
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