Association of AMPK and eNOS gene polymorphisms with endurance exercise performance

• Main Authors: Yi-Ting Chen, 陳怡廷
• Other Authors: Shen-Yu Hsieh
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/77395342629313502417

碩士 === 國立臺灣師範大學 === 體育學系 === 97 === AMPK can be activated by exercise, muscle contraction and hypoxia to regulate various cellular metabolic pathways. AMPK is able to stimulate glucose uptake in skeletal muscle by phosphorylating eNOS and increase NO production. Also, genetic variants of AMPK and eNOS may affect metabolic regulation. However, there were little studies connect AMPK and eNOS to exercise performance. Purpose: To investigate the difference of PRKAA2 and eNOS gene polymorphism in elite endurance athletes and healthy control group. Methods: 103 endurance athletes (swimming, rowing, cycle, soccer, rugby, and tennis) who had won third place and above in national competitions were as the experimental group. The control group was 200 healthy people drawn from Taiwanese general population. The genotype of PRKAA2 (rs857155) was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), the genotype of eNOS intron 4 a/b was determined by polymerase chain reaction (PCR), and the genotype of eNOS G894T was determined by primer extension with denaturing high performance liquid chromatography (DHPLC). Data were analyzed with SPSS software version 12.0. χ2-test was employed to evaluate the difference (α= .05). Results: The frequency of the PRKAA2 (rs857155) C allele was significantly higher in tennis and rowing athletes as compared to the controls. The distribution of eNOS intron 4 a/b genotype and allele frequencies was significantly different between the athletes (aa/ab/bb = 2.9%/26.2%/70.9%; a/b = 16.0%/84.0%) and the controls (aa/ab/bb = 1.5%/15.5%/83.0%; a/b = 9.3%/90.8%), this phenomenon was also seen in male athletes. There was also a significant difference between the cycle athletes and the controls as well as the rowing athletes and the controls, with more tennis and rowing athletes carrying the a allele. Significant linkage disequilibrium was found between eNOS intron 4 a/b and eNOS G894T. The frequency of the a allele (aa+ab) and the T allele (GT+TT) combination was significantly higher in the athletes than in the controls. Conclusion: eNOS intron 4 a/b is associated with endurance exercise performance, and it might be a candidate gene for searching elite endurance athletes.