| Summary: | 碩士 === 國立臺灣海洋大學 === 海洋生物研究所 === 97 === In this report a new thermophilic anaerobic bacterium Thermoanaerobacterium sp. NTOU2 from Gueishan island which was capable of growth utilizing hexose and pentose at 70 ℃ was used to investigate its potential for ethanol production/yield from sugars. When using 10 mM KH2PO4/K2HPO4+9 g/L NaHCO3–CO2 as buffer in medium, the growth of strain NTOU2 were faster than other buffer. When the strain NTOU2 using 0.5 % starch or 0.5 % glucose + 0.5 % cellobiose as the substrate, the ethanol production were highest than other sugar. When using 0.5 % glucose and 0.5 % xylose as the substrate, the highest ethanol production by strain NTOU2 is at pH 6.5. The highest ethanol production was found in the medium containing 0.5 % glucose + 0.5 % yeast extract, 0.5 % xylose + 0.5 % yeast, 1.0 % starch and 1.0 % yeast. The presence of 0.75 % or higher concentration of 5-HMF, 0.5 % or higher concentration of levulinic acid, 0.05 % or higher concentration of furfural, 0.5 % or higher concentration of formic acid, and 0.3 % or higher concentration of acetic acid sharply inhibited the growth and ethanol production by strain NTOU2 when 0.5 % glucose was used as the substrate. The presence of 0.5 % or higher concentration of 5-HMF, 0.5 % or higher concentration of levulinic acid, 0.1 % or higher concentration of furfural, 0.5 % or higher concentration of formic acid, and 0.4 % or higher concentration of acetic acid sharply inhibited the growth and ethanol production by strain NTOU2 when 0.5 % xylose was used as the substrate.
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