The intranasal delivery study of liposomal-vaccine to SPF chickens

• Main Authors: Li-Ping Tseng, 曾莉萍
• Other Authors: 黃義侑
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/62439441115507741770

博士 === 國立臺灣大學 === 醫學工程學研究所 === 97 === Liposomal-NDV vaccine intranasal administration to chicken can induce specific-NDV antibody at mucosal and serum. The adjuvant effect of multi-lamellar vehicles (MLVs) liposomes formulated with three phospholipids including phosphatidylcholine-liposomes (PC-Lip), phosphatidylserine-liposomes (PS-Lip), and stearylamine-liposomes (SA-Lip) was compared with that for virus alone using the inactivated Newcastle disease virus (NDV) as model antigen. PC-Lip and PS-Lip induced more NDV antibody titer after secondary immunizations. In response to virulent viral challenge, all control animals died, whereas 80~90% of animals which still survived. Added LPS with liposomal-NDV vaccine which enhanced chickens produced antibody in serum and nasal/tracheal lavages. Furthermore, we combined tremella with liposomal-NDV vaccine to enhance the muco-adhesive property and prolong liposomes on the mucosal surface. Chickens received T-PC-Lip resulted in a significant increase in HI titer also elicited a significant mucosal secretary immunoglobulin A (s-IgA) response in tracheal lavages and a serum IgG response. After virulent virus challenge, the protection rate of T-PC-Lip vaccine showed 100% survival rate. The molecular mechanism of liposomal-adjuvant was study both in vitro and in vivo. Confocal laser scan microscopy showed that PC-Lip were uptaken into chicken spleen macrophages. PC-Lip and PS-Lip stimulated chickens spleen macrophages produce NO. In response to PC-Lip stimulated, Bay 11-7085 and U0126 inhibited macrophages produced NO. PC-Lip was shown to be involved in both activation of NF-κB and upstream ERK of MEK pathway. Moreover, PC-Lip and T-PC-Lip were shown to be activated ERK pathway in vovo. In conclusion, T-PC-Lip is an active adjuvant for chickens resulting in trigger Th1 pathway of cellular immunity and Th2 pathway of humoral immunity.