1.Chemical constituents from Senecio scandens2.Characterization of antifungal principles from the tea seed pomace

• Main Authors: Chih-Lung Lin, 林志龍
• Other Authors: Ping-Chung Kuo
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/32tpf8

碩士 === 國立虎尾科技大學 === 光電與材料科技研究所 === 97 === Senecio scandens Buch. -Ham (Asteraceae) is a Formosan Senecio species. This genus is widely distributed throughout the world and comprises more than 1500 species. There are about 20 species in Taiwan and the researches regarding this species were few. Therefore S. scandens was selected as a target for investigations of its phytochemical diversity. The methanol extracts of whole plants of S. scandens were fractionated into alkaloid, chloroform, and water solubles, successively, and further purified using silica gel column chromatography, reversed-phase Diaion HP-20 column chromatography, thin-layer chromatography, and high performance liquid chromatography, respectively. Forty compounds were characterized from the crude extracts of S. scandens and among them compound (1) was a new compound reported for the first time. Furthermore, the isolated lignans and steroids with more quantities were subjected into the examination for their antioxidant activities by DPPH free radical scavenging assay. The results displayed that some of the isolates exhibited significant antioxidant bioactivity and maybe valuable to be evaluated further. The tea seed pomace is the residue of the seeds of Camellia oleifera after the oil was pressed from the seeds, and it was reported to possess the inhibitory bioactivity of the growth of Rhizoctonia solani kühn AG-4. However, the active principles were remained unknown, so that the present study was aimed to characterize the antifungal constituents in the methanolic extracts of the tea seed pomace. The methanol crude extract of tea seed pomace was purified using reversed-phase column chromatography and high performance liquid chromatography with the aid of bioassay guided fractionation. Totally two flavonoids and one saponin mixture were characterized from the tea seed pomace and were subjected into the examination of the antifungal activity. It could help to determine the active principle and discover new biocontrol sources.