| Summary: | 碩士 === 靜宜大學 === 食品營養研究所 === 97 === Tempeh is a traditional fermentative food in Indonesia. The bio-functions of tempeh including antioxidant activity, cholesterol lowing, preventing progress of several cancers and antimicrobial activity. This study aimed to evaluate the antimicrobial activity and cytotoxicity toward mammalian cancer cell of water extract of tempeh; furthermore, the effect of tempeh on the alteration of cecal microbiota and also their inhibitory effect on the precancerous colon lesions (aberrant crypt foci, ACF) in male SD (Sprague Dawley) rats were assessed. The paper disc diffusion test was performed to assess the effect of water extract of tempeh on the growth of pathogenic bacteria, including Salmonella typhimurium, Staphylococcus aureus, Listera monocytogenes, Psuedomonas aeruginosa, Propionibacterium freudenreichii and Escherichia coli. Cytotoxicity was detected by MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay) assay using human Caco-2 colon adenocarcinoma cell. Effect of tempeh on anticoloncancer was evaluated in SD (Sprague Dawley) rats. After induced ACF by 1, 2-Dimethylhydrazine Dihydrochloride (DMH), rats were gavaged with either soybean or tempeh at a daily dosage of 300 mg/kg BW or 600 mg/kg BW for 12 weeks. At the end of experiment, colons were collected and analyzed for cecal microbiota, cecal pH, short chain fatty acid composition, ACF formation and crypt multiplicity; on the other hand, blood biochemical parameters and antioxidant activitues were determined as well. The results of this study showed that water extract of tempeh could inhibit the growth of L. Monocytogenes and Caco-2 cell. Oral administration of soybean or tempeh did not affect on body weight and food intake in SD rats. Both soybean and tempeh could decrease the number of C. perfugens in cecal content compared to DMH-induced group. SD rats were gavaged with 300 mg/kg BW soybean could increase superoxide dismutase activity; however, this phenomenon did not appear in the rats fed with equal dosage of tempeh. Both soybean and tempeh significantly reduced the number of aberrant crypt foci (ACF) in the colon of DMH-treated rat (p<0.05). Oral administration of 300 mg/kg BW soybean or 600 mg/kg BW tempeh could reduce 50 % of tatol number of ACF. Especially, daily intake tempeh 600 mg/kg BW could reduce number of ACF which was composed by more than 4 crypts. These results suggested that soybean and tempeh supplementation may be beneficial to inhibit colon cancer by elevating the SOD activity of liver tissue or lowering β-Glucuronidase activity in cecal through inhibiting the growth of C.perfringens.
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