Production of acid protease from Aspergillus sp. by solid-state fermentation
碩士 === 南台科技大學 === 生物科技系 === 97 === Acid protease is a kind of hydrolase with wide applications in food processing、medical purpose、leather processing and feeds. Acid protease having stability in acidic and neutral conditions can adapt digestion systems of animals, which can increase the digestion and...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2009
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| Online Access: | http://ndltd.ncl.edu.tw/handle/48017759367014037169 |
| Summary: | 碩士 === 南台科技大學 === 生物科技系 === 97 === Acid protease is a kind of hydrolase with wide applications in food processing、medical purpose、leather processing and feeds. Acid protease having stability in acidic and neutral conditions can adapt digestion systems of animals, which can increase the digestion and absorption rate of feed nutrients. The fungal strain Aspergillus sp. is capable of producing an acid protease that can be used as a feed additive.
In the study, we found that wheat bran could be used as the major solid substrate. NH4H2PO4, NH4NO3 and soya milk all were the significant effect factors and could obviously increase the production of acid protease. Temperature and moisture were also the important fermentation parameters for the solid state fermentation.
The solid-state fermentation of Aspergillus sp. was optimized to produce the acid protease by using Taguchi methods. The optimal level of media compositions obtained by orthogonal arrays included wheat bran 10 g、NH4H2PO4 3%、NH4NO3 1%、soya milk 10 ml、initial moisture 120% and culture temperature at 26℃, which apparently increased the acid protease activity to 51.54U/g.
80% of the crude acid protease can be recovery from crude extract by ammonium sulfate fractionation between ammonium sulfate percent saturation concentrations from 60% to 90%.
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