Inhibitory effect of the 50% ethanol extract of Solanum lyratum Thunb. on tyrosinase activity

• Main Authors: Ming-Hsiung Chiu, 邱敏雄
• Other Authors: Cheng-Kuang Hsu
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/18002609607861083089

碩士 === 亞洲大學 === 生物科技學系碩士班 === 97 === This study aimed to evaluate the inhibition properties of 50% ethanol extract from Solanum lyratum Thunb. (SLEE) toward the activity of tyrosinase. When using L-3,4-dihydroxyphenylalanine (L-dopa) as the substrate for mushroom tyrosinase, SLEE showed inhibitory effect on the activity of mushroom tyrosinase. SLEE decreased the value of Vmax and increased Km, and it behaved as a mixed-type inhibitor toward mushroom tyrosinase. Human malignant melanoma A375.S2 cell line was also used to evaluate the influence of SLEE on tyrosinase activity in the cell. It was found that SLEE reduced the tyrosinase activity in the A375.S2 cells at medium and high enzyme substrate levels (L-dopa, 0.5 and 1.0 mg/ml, respectively), but not at low enzyme substrate level (0.1 mg/ml). It was concluded that SLEE might be a potential inhibitor of tyrosinase.