Determination of Binding Constants for Polyphenols and Borates

• Main Authors: Ya-Ting Chang, 張雅婷
• Other Authors: 黃玲媛
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/3wgu5w

碩士 === 國立臺北科技大學 === 化學工程研究所 === 97 === In this article, we determine binding constants and binding stoichiometry for polyphenols compound and borates by capillary electrophoresis with viscosity correction , current correction and job’s method of ultraviolet-visible spectroscopic. Compare with the binding stoichiometry for polyphenols compound and borates by Job’s method,binding stoichiometry for polyphenols compound (Wogonin、Luteolin、Apigenin、Naringenin ) to borates by capillary electrophoresis with viscosity correction and current correction show good agreement with 1：2 , which is the same as the result by Job’s method. The binding constant of polyphenols compound and borates：Baicalin (1.64×105)，Wogonin(2.10×105)，Luteolin(1.93×105)，Apigeni(1.51×105)，Naringenin(1.94×105)，Hispidulin(7.40×104)，Protocatechuic acid (3.27×107)，p-Coumaric acid(1.16×108). So, the binding constant of polyphenols compound and borates is bigger than far flavonoids compound and borates.