| Summary: | 碩士 === 臺北醫學大學 === 生藥學研究所 === 97 === Gliomas are the most common type of primary brain tumor. Nearly two-thirds of gliomas are highly malignant lesions that account for a disproportionate shape of brain tumor-related morbidity and mortality. Approximately 17,000 primary brain tumors are diagnosed every year, and of those, about 60% are gliomas. Anaplastic astrocytoma (AA, grade III) and glioblastoma multiforme (GBM, grade IV), the high grade gliomas, are the most common type hence we employed the human malignant glioma cell lines of U87 MG (III) and GBM 8401 (IV) as target to examined antiproliferation activity by MTT reduction.
Brain tumor therapy usually gets surgical resection for the first priority and followed by radiotherapy and chemotherapy. All brain tumor therapies now usually result in the poor prognosis. Especially, radiotherapy and chemotherapy would generate free radicals, kill not only cancer cells but also healthy cells. Recent studies have demonstrated that antioxidant could enhance the therapeutic effects, decrease the side effects and protect normal tissue damage. Phytochemicals are plant-derived compounds under scientific research for potential health promoting properties and recurrence of cancer. Epidemiological and preclinical evidence suggests that polyphenolic phytochemicals possess cancer chemopreventive properties.
In this study, a high through screening method was established to investigate 60 kinds of natural botany resource on anti-brain tumor activity. YY-02 methanol extract (ME) is the most efficacy of antiproliferation on human malignant glimoas. Furthermore, ME was fractionated with n-hexane (HeF), ethyl acetate (EA), acetone (AcF) and methanol (MeF). Outcomes of total polyphenol, flavonoid content, and DPPH radical scavenged, chelated ferrous ion, lipid peroxidation (LPO) inhibition of liver, kidney and brain tissue measurement, that exerted the MeF is a highest potential fraction in YY-02. Otherwise, view of morphological assay and cell cycle analysis, YY-02 exhibited the cell death pathway by apoptosis. In conclusion, YY-02 is a phytochemical of chemotherapy agents on brain tumor.
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