STRUCTRURE GENE ANALYSIS OF ASPERGILLUS NIGER ENDOGLUCANASE B AND CELLUBIOHYDROLASE A

• Main Authors: Hsing-Ren Wang, 王興仁
• Other Authors: Trong-Rong Yan
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/38070499680139155789

碩士 === 大同大學 === 生物工程學系(所) === 97 === Cellulose is the most abundant and renewable biopolymer on Earth. Cellulose has been used for centuries; however, it's enormous potential as a renewable source of energy was recognized only after the cellulose degrading enzymes or "cellulases" had been identified. Microorganisms including fungi, bacteria and actinomycetes produce mainly three types of cellulase: endoglucanase, exoglucanase and β-glucosidase. Aspergillus niger can be used in function food and pharmaceutical industrial. For the purpose of enzyme cloning, propertise enhancement, and regulation comprehension; we designed primers from known endoglucanase and cellobiohydrolase sequence and succeed in obtaining the DNA and RNA-reversed transcribed cDNA sequence of A. niger endoglucanase and cellobiohydrolase. The endoglucanase B cloned genomic sequence revealed a 1,217 bp open reading frame with 5 introns and 6 exons. The cDNA showed a 996 bp sequence encoding an 331 amino acid protein that belongs to glycosyl hydrolase family 5. The full length of cellobiohydrolase A is 1,512 bp. Its cDNA shows 1,359 bp. There are 3 intron in the cbhA gene. The CBHA belongs of glycosyl hydrolase 7.