STRUCTRURE GENE ANALYSIS OF ASPERGILLUS NIGER ENDOGLUCANASE B AND CELLUBIOHYDROLASE A
碩士 === 大同大學 === 生物工程學系(所) === 97 === Cellulose is the most abundant and renewable biopolymer on Earth. Cellulose has been used for centuries; however, it's enormous potential as a renewable source of energy was recognized only after the cellulose degrading enzymes or "cellulases" had...
Main Authors: | , |
---|---|
Other Authors: | |
Format: | Others |
Language: | zh-TW |
Published: |
2009
|
Online Access: | http://ndltd.ncl.edu.tw/handle/38070499680139155789 |
id |
ndltd-TW-097TTU05106010 |
---|---|
record_format |
oai_dc |
spelling |
ndltd-TW-097TTU051060102016-05-02T04:11:10Z http://ndltd.ncl.edu.tw/handle/38070499680139155789 STRUCTRURE GENE ANALYSIS OF ASPERGILLUS NIGER ENDOGLUCANASE B AND CELLUBIOHYDROLASE A 黑麴菌內切型纖維素酵素B與外切型纖維素酵素A基因選殖與結構分析 Hsing-Ren Wang 王興仁 碩士 大同大學 生物工程學系(所) 97 Cellulose is the most abundant and renewable biopolymer on Earth. Cellulose has been used for centuries; however, it's enormous potential as a renewable source of energy was recognized only after the cellulose degrading enzymes or "cellulases" had been identified. Microorganisms including fungi, bacteria and actinomycetes produce mainly three types of cellulase: endoglucanase, exoglucanase and β-glucosidase. Aspergillus niger can be used in function food and pharmaceutical industrial. For the purpose of enzyme cloning, propertise enhancement, and regulation comprehension; we designed primers from known endoglucanase and cellobiohydrolase sequence and succeed in obtaining the DNA and RNA-reversed transcribed cDNA sequence of A. niger endoglucanase and cellobiohydrolase. The endoglucanase B cloned genomic sequence revealed a 1,217 bp open reading frame with 5 introns and 6 exons. The cDNA showed a 996 bp sequence encoding an 331 amino acid protein that belongs to glycosyl hydrolase family 5. The full length of cellobiohydrolase A is 1,512 bp. Its cDNA shows 1,359 bp. There are 3 intron in the cbhA gene. The CBHA belongs of glycosyl hydrolase 7. Trong-Rong Yan 顏聰榮 2009 學位論文 ; thesis 81 zh-TW |
collection |
NDLTD |
language |
zh-TW |
format |
Others
|
sources |
NDLTD |
description |
碩士 === 大同大學 === 生物工程學系(所) === 97 === Cellulose is the most abundant and renewable biopolymer on Earth. Cellulose has been used for centuries; however, it's enormous potential as a renewable source of energy was recognized only after the cellulose degrading enzymes or "cellulases" had been identified.
Microorganisms including fungi, bacteria and actinomycetes produce mainly three types of cellulase: endoglucanase, exoglucanase and β-glucosidase. Aspergillus niger can be used in function food and pharmaceutical industrial. For the purpose of enzyme cloning, propertise enhancement, and regulation comprehension; we designed primers from known endoglucanase and cellobiohydrolase sequence and succeed in obtaining the DNA and RNA-reversed transcribed cDNA sequence of A. niger endoglucanase and cellobiohydrolase. The endoglucanase B cloned genomic sequence revealed a 1,217 bp open reading frame with 5 introns and 6 exons. The cDNA showed a 996 bp sequence encoding an 331 amino acid protein that belongs to glycosyl hydrolase family 5. The full length of cellobiohydrolase A is 1,512 bp. Its cDNA shows 1,359 bp. There are 3 intron in the cbhA gene. The CBHA belongs of glycosyl hydrolase 7.
|
author2 |
Trong-Rong Yan |
author_facet |
Trong-Rong Yan Hsing-Ren Wang 王興仁 |
author |
Hsing-Ren Wang 王興仁 |
spellingShingle |
Hsing-Ren Wang 王興仁 STRUCTRURE GENE ANALYSIS OF ASPERGILLUS NIGER ENDOGLUCANASE B AND CELLUBIOHYDROLASE A |
author_sort |
Hsing-Ren Wang |
title |
STRUCTRURE GENE ANALYSIS OF ASPERGILLUS NIGER ENDOGLUCANASE B AND CELLUBIOHYDROLASE A |
title_short |
STRUCTRURE GENE ANALYSIS OF ASPERGILLUS NIGER ENDOGLUCANASE B AND CELLUBIOHYDROLASE A |
title_full |
STRUCTRURE GENE ANALYSIS OF ASPERGILLUS NIGER ENDOGLUCANASE B AND CELLUBIOHYDROLASE A |
title_fullStr |
STRUCTRURE GENE ANALYSIS OF ASPERGILLUS NIGER ENDOGLUCANASE B AND CELLUBIOHYDROLASE A |
title_full_unstemmed |
STRUCTRURE GENE ANALYSIS OF ASPERGILLUS NIGER ENDOGLUCANASE B AND CELLUBIOHYDROLASE A |
title_sort |
structrure gene analysis of aspergillus niger endoglucanase b and cellubiohydrolase a |
publishDate |
2009 |
url |
http://ndltd.ncl.edu.tw/handle/38070499680139155789 |
work_keys_str_mv |
AT hsingrenwang structruregeneanalysisofaspergillusnigerendoglucanasebandcellubiohydrolasea AT wángxìngrén structruregeneanalysisofaspergillusnigerendoglucanasebandcellubiohydrolasea AT hsingrenwang hēiqūjūnnèiqièxíngxiānwéisùjiàosùbyǔwàiqièxíngxiānwéisùjiàosùajīyīnxuǎnzhíyǔjiégòufēnxī AT wángxìngrén hēiqūjūnnèiqièxíngxiānwéisùjiàosùbyǔwàiqièxíngxiānwéisùjiàosùajīyīnxuǎnzhíyǔjiégòufēnxī |
_version_ |
1718253344080789504 |