| Summary: | 碩士 === 大同大學 === 生物工程學系(所) === 97 === It’s well known that bacteria have ability to develop antibiotic-resistance in short time after treatment. To find therapeutic agents with different mode of action than conventional antibiotics is a necessity. To meet the above requirement, antimicrobial peptides (AMPs) represent a good alternative for infective antibiotics. AMP LL-37 with positive charges belongs to an immune system that has a capacity to distinguish membrane from different lipids in human. This special property makes LL-37 destroy the negative charged bacteria membranes. Therefore the above mechanism is effective to drug resistance bacteria. Normally bacteria and LL-37 will not exist in urine, except for those patients who suffer urinary tract infection (UTI). Hopefully, a screening biosensor can be made by using the above character.
DSC (Differential Scanning Calorimeter) was used to measure interaction between
LL-37 and liposome that plays a biomembrane role. The stability of the complex can be affected by adding interference salt and urea. In the meantime, the change of liposome structure can be observed by a fluorescence probe on it. Finally, the biosensor was made by immobilizing liposomes on silane coated surface. Testing was made by mixing the sample containing LL-37 on the biosensor plate. The content of LL-37 can be detected and determined by impedance and phase angle variations.
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