| Summary: | 碩士 === 國立陽明大學 === 生醫光電工程研究所 === 97 === The studies in this thesis are twofold. In the first part, we are investigating the fluorescence resonant energy transfer (FRET) between quantum dots and gold nanorods as the donor and the acceptor, respectively. The FRET are examined by varying the spectral overlapping and the distance between the donors and the acceptors. The emission peak of the quantum dots is at 605 nm. Gold nanorods with absorption peaks located at 650, 700, and 750 nm are attached to the quantum dots. The FLIM measurements show that the magnitude of lifetime decrement, indicating the extent of FRET, increases with the spectral overlapping. Te gold nanorods are also coated with polymer with thickness of 10 and 15 nm to vary the interacting distance between the quantum dots and the gold nanorods. The FRET efficiency is found to decrease to 1% when the distance is increased to beyond 10nm. These two measurements show that the quantum dot can transfer the energy to the gold nanorods by resonance.
Additionally, we have successfully developed a spectrally resolved module for the fluorescence lifetime imaging microscopy platform. It is used to correlate the lifetime and the fluorescence spectrum. As somewhat expected, we found that the lifetime and the FRET efficiency are similar at the various parts of the emission spectrum.
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