| Summary: | 碩士 === 國立陽明大學 === 生醫光電工程研究所 === 97 === Abstract
Surface Plasmon Resonance has got lots of attention in last few years. It provides label-free and high surface sensitivity. However, decreasing the cost, high-throughput detection, and improving sensitivity are the main trends for everyone wants to achieve.
In this the thesis, I fabricate the period nanostructure on silicon subtract by E-beam lithography and RIE etching and then using the nanoinprint technology to replcate the nanostructure to glass subtract. In this way, it can not only decrease the time for fabricating the nanostructure sample but also the cost. The sensitivity of nanostructure base chip is done by glycerol solution in diffirent concentration. The result shows the sensitivity has no significant different performance between wavelength detection (3.1×10-5) and intensity detection (8.5×10-5) ,however, the intensity detection has the potential for high-throughput sensing development.
In the protein array sensing experiment, BSA and anti-BSA as the demonstration of molecular interaction dynamic detection, the BSA concentration is 7 nM and anti-BSA is 2.5 nM. The wavelength in 846 nm of LED as the light source in this experiment setup. The CCD records the image and analysising the image to get the intensity changing during the experiment. In the end, I perform the three slit array detection result. The anti-BSA detecting limitation is 35pM in assuming the light stability is 0.2%.
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