Immunomodulatory and Anti-inflammatory Effects of Polysaccharides from Mycelia and Fermented Culture Broth of Antrodia camphorata

• Main Authors: Chiao-Chan Wang, 王喬嬋
• Other Authors: John Chin Tsaihong
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/84052611444094936842

碩士 === 國立陽明大學 === 臨床醫學研究所 === 97 === Antrodia camphorata grows on characterized Cinnamomum kanehirai in Taiwan, it has become the source of health food in recent years. A. camphorata has anticancer, antitumor and immunomodulatory properties. In this study, BALB/c mice were fed with polysaccharides obtained from fermented culture broth and mycelia of A. camphorata at different time points to investigate the anti-inflammatory action and the immunomodulatory effect. When mice were administrated with two kinds of polysaccharides for a period of time and then injected LPS into mice, we found that Trem-1HI expression on macrophages is inhibited measured by flow cytometry. This result indicates that A. camphorata has anti-inflammatory action. The expressions of CD40HI and CD8αHI markers on DCs and CD8αHI marker on macrophages increased apparently. However, the CD86 marker expressed on DCs decreased sharply, this result may regulated by the activation of CTLA-4 and ICOS from T cells. The TNF-α、IFN-γ、IL-4、IL-6、IL-10 and IL-12 mRNA cytokines inhibited obviously after feeding with A. camphorata by RT-PCR. In addition, we use real-time PCR to detect Trem-1、IL-1β and iNOS cytokine content and profile; also, to observe the expression of Trem-1 protein with western blot. The results demonstrated that two kinds of polysaccharides from A. camphorata possesses effects on both immunomodulation and anti-inflammation. These markers may serve as targets for further understanding the molecular basis and efficacy of A. camphorata and other Chinese herbal medicines in the future.