| Summary: | 碩士 === 國立陽明大學 === 醫學生物技術暨檢驗學系暨研究所 === 97 === Embryonic stem cells (ESCs) possess the ability to undergo multiple self-renewal cycles whilst maintaining their pluripotent state. The pluripotency and self-renewal of ESCs are regulated by a variety of cytokines/growth factors. Recent study showed that the zinc-finger protein-Zfx impaired the self-renewal of ES cells. It was indicated that T-cell leukimia 1 (Tcl-1) could be the downstream effector of Zfx. Thus we initially attempted to determine whether Tcl-1 is directly regulated by Zfx though AKT pathway. First, we showed that inhibit PI3K/Akt pathway would downregulate Nanog, Oct4, and possibly Tcl-1, but not Zfx. Addition, overexpression of Zfx did not affect the level of Tcl-1 in ESCs indicating that Tcl-1 might not be a direct target of Zfx. However, we demonstrated showed overexpression of Tcl-1 indeed upregulated the level of Oct4 indicating a feedback role of Tcl-1 in maintenance of ESC pluripotency. We also confirmed reduced level of Zfx did not affect expression level of Oct4 and Nanog, and the self-renewal growth of mouse ESCs by condition knockdown system with ShRNA. Taken together, Zfx may regulate self-renewal of embryonic stem cells though other pathway.
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