Nod2 Mutation Enhances NF-kappaB activity and Bacterial Killing Activity of Macrophages

碩士 === 國立陽明大學 === 急重症醫學研究所 === 97 === NOD2, an intracellular sensor of bacteria, are linked to increased susceptibility to bacteria in Crohn’s disease (CD). The NOD2 protein is expressed mainly by macrophages and dendritic cells. This study is to examine the role of NOD2 in the innate response of ma...

Full description

Bibliographic Details
Main Authors: Chien-Jen Chang, 張建仁
Other Authors: Lee-Wei Chen
Format: Others
Language:zh-TW
Published: 2009
Online Access:http://ndltd.ncl.edu.tw/handle/58112259413866529028
id ndltd-TW-097YM005798006
record_format oai_dc
spelling ndltd-TW-097YM0057980062016-05-04T04:16:42Z http://ndltd.ncl.edu.tw/handle/58112259413866529028 Nod2 Mutation Enhances NF-kappaB activity and Bacterial Killing Activity of Macrophages Nod2基因突變增強巨噬細胞的NF-kappaB活動及殺菌活動 Chien-Jen Chang 張建仁 碩士 國立陽明大學 急重症醫學研究所 97 NOD2, an intracellular sensor of bacteria, are linked to increased susceptibility to bacteria in Crohn’s disease (CD). The NOD2 protein is expressed mainly by macrophages and dendritic cells. This study is to examine the role of NOD2 in the innate response of macrophages to bacterial challenge. First, peritoneal macrophages and alveolar macrophages were harvested from WT, Nod22939iC, as well as TLR4-/- mice and incubated with E. coli or P. aeruginosa. Bacterial killing activity; IL-1b and TLR4 protein expression; NF-kB DNA binding activity assay; as well as IL-1b, TNFa, TLR2, TLR4 and TLR9 mRNA expression of macrophages were examined. We found that alveolar macrophages and peritoneal macrophages of Nod22939iC mice but not WT mice or TLR4-/- mice demonstrated a significant increase of E. coli killing activity. Bacterial challenge also induced a significant increase of pro-IL-1b protein expression; NF-kB DNA binding activity; as well as IL-1b and TNFa mRNA expression of the peritoneal macrophages in Nod22939iC mice. Colletively, the increase of bacterial killing activity, IL-1b expression, and NF-kB DNA binding activity of macrophages in Nod22939iC mice suggests that NOD2 is a positive regulator of NF-kB/IL-1b-mediated innate response to bacteria challenge in Crohn’s disease. Nod22939iC also cause increased bacterial killing activity in macrophage of mice. Lee-Wei Chen 陳理維 2009 學位論文 ; thesis 62 zh-TW
collection NDLTD
language zh-TW
format Others
sources NDLTD
description 碩士 === 國立陽明大學 === 急重症醫學研究所 === 97 === NOD2, an intracellular sensor of bacteria, are linked to increased susceptibility to bacteria in Crohn’s disease (CD). The NOD2 protein is expressed mainly by macrophages and dendritic cells. This study is to examine the role of NOD2 in the innate response of macrophages to bacterial challenge. First, peritoneal macrophages and alveolar macrophages were harvested from WT, Nod22939iC, as well as TLR4-/- mice and incubated with E. coli or P. aeruginosa. Bacterial killing activity; IL-1b and TLR4 protein expression; NF-kB DNA binding activity assay; as well as IL-1b, TNFa, TLR2, TLR4 and TLR9 mRNA expression of macrophages were examined. We found that alveolar macrophages and peritoneal macrophages of Nod22939iC mice but not WT mice or TLR4-/- mice demonstrated a significant increase of E. coli killing activity. Bacterial challenge also induced a significant increase of pro-IL-1b protein expression; NF-kB DNA binding activity; as well as IL-1b and TNFa mRNA expression of the peritoneal macrophages in Nod22939iC mice. Colletively, the increase of bacterial killing activity, IL-1b expression, and NF-kB DNA binding activity of macrophages in Nod22939iC mice suggests that NOD2 is a positive regulator of NF-kB/IL-1b-mediated innate response to bacteria challenge in Crohn’s disease. Nod22939iC also cause increased bacterial killing activity in macrophage of mice.
author2 Lee-Wei Chen
author_facet Lee-Wei Chen
Chien-Jen Chang
張建仁
author Chien-Jen Chang
張建仁
spellingShingle Chien-Jen Chang
張建仁
Nod2 Mutation Enhances NF-kappaB activity and Bacterial Killing Activity of Macrophages
author_sort Chien-Jen Chang
title Nod2 Mutation Enhances NF-kappaB activity and Bacterial Killing Activity of Macrophages
title_short Nod2 Mutation Enhances NF-kappaB activity and Bacterial Killing Activity of Macrophages
title_full Nod2 Mutation Enhances NF-kappaB activity and Bacterial Killing Activity of Macrophages
title_fullStr Nod2 Mutation Enhances NF-kappaB activity and Bacterial Killing Activity of Macrophages
title_full_unstemmed Nod2 Mutation Enhances NF-kappaB activity and Bacterial Killing Activity of Macrophages
title_sort nod2 mutation enhances nf-kappab activity and bacterial killing activity of macrophages
publishDate 2009
url http://ndltd.ncl.edu.tw/handle/58112259413866529028
work_keys_str_mv AT chienjenchang nod2mutationenhancesnfkappabactivityandbacterialkillingactivityofmacrophages
AT zhāngjiànrén nod2mutationenhancesnfkappabactivityandbacterialkillingactivityofmacrophages
AT chienjenchang nod2jīyīntūbiànzēngqiángjùshìxìbāodenfkappabhuódòngjíshājūnhuódòng
AT zhāngjiànrén nod2jīyīntūbiànzēngqiángjùshìxìbāodenfkappabhuódòngjíshājūnhuódòng
_version_ 1718256013296009216