Bioreactor development and fermentative strategies for bioethanol production using the process of simultaneous saccharification and fermentation

• Main Authors: Yuching-Huang, 黃郁菁
• Other Authors: Yu-Hong Wei
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/82128897355357038050

碩士 === 元智大學 === 生物科技與工程研究所 === 97 === In this study, we developed a novel bioreactor that simultaneously enhanced the saccharification and fermentation process for bioethanol production. Initially, A. niger and T. reesei are co-immobilized on PU (polyurethane) carrier, and co-culture in our bioreactor by using Carboxymethylcellulose (CMC) as substrate, then Z. mobilis alginate beads were added into this bioreactor to overcome the problem of simultaneous saccharification and bioethanol fermentation. We show that the yield of the bioethanol could obtain 553.75 mg/L at 21 hours, when we added 10 g/L CMC in this co-culture system. It has recently been reported that the maximum ethanol concentration (777.8 mg/L) increased nearly 1.40-fold when the CMC concentration was increased from 10 to 15 g/L. In order to predict the possibility of agriculture wastes (such as napier grass and rice straw) replace CMC to produce bioethanol by the novel bioreactor. We found that the concentration of ethanol could obtain 223.67 mg/L at 13 hour under the napier grass (10 g/L) was used as the carbon source. Furthermore, the ethanol concentration (507.44 mg/L) increased nearly 2.27-fold when the napier concentration was increased from 10 to 15 g/L. In future, this modified bioreactor will be used to scale-up to insure the bioethanol industry.