Silica confined ionic liquid stationary phases for high performance liquid chromatography and supercritical fluid chromatography

• Main Authors: Ming-En Yu, 余銘恩
• Other Authors: Guor-Tzo Wei
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/82402460437413680244

碩士 === 國立中正大學 === 化學所 === 98 === In pharmaceutics, the capability of doing simultaneous separation of multiple active ingredients of drugs in a separation is getting important for drug analysis. In this work, the nature of multiple-modes interaction of silica based ionic liquids was utilized to create highly selective stationary phases for simultaneous separation of different types of ingredients by chromatographic techniques. Three HPLC stationary phases, SiO2-[VBBI][Cl]、SiO2- [C9(vim)2] [Br]2-[VBBI][Cl]、and SiO2-[DVB-VBBI][Cl], were prepared by covalently bonding 1-vinylbenzyl-4-butylimidazole (VBBI), divinyl -nonanalimidazole (C9(vim)2), and divinylbenzene (DVB) onto the silica gel. The molecular interactions of hydrophobic, polar and π-π interactions of the stationary phases were examined by the retention behaviors of neutral (polycyclic aromatic hydrocarbons, PAHs) and polar (steroids) compounds. In addition, the potential of using these stationary phases for per-aqueous liquid chromatography (PALC) and hydrophilic interaction liquid chromatography (HILIC) separations were evaluated from the separation of alkaloids. In addition, stationary phase, the separations of acidic (nonsteroidal anti-inflammatory drugs, NSAIDs), basic (cold medicines), and neutral (PAHs and steroids) compounds with SiO2-DVB-[VBBI][Cl] by supercritical liquid chromatography (SFC) was studied. The effects of various parameters of SFC on the separation of these compounds were examined. The data indicate that the separation mechanism appears to involve multiple interactions: including electrostatic, hydrophobic, hydrogen bonding, and π-π interactions between the analytes and the ionic liquid (IL)-modified stationary phase in SFC. The multiple-mode interaction enables the effective separation of acidic, basic and neutral drugs by SFC with these stationary phases. The multiple-mode interaction seems to give these columns better separation performance than those of commercial columns for the separation of acidic, basic, and neutral compounds.