Detection of Meningitis-inducing Bacteria by Multiplex PCR

• Main Authors: Chi-Tsung Li, 李奇聰
• Other Authors: Kuang-Ming Hsiao
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/35978073313404144684

碩士 === 國立中正大學 === 生命科學系暨分子生物研究所暨生物醫學研究所 === 98 === Acute bacterial meningitis is a disease with high mortality rate in the developed and developing countries. The clinical symptoms, including high fever, vomiting, and lethargy, are not disease-specific and the bacteria that associate with adult bacterial meningitis are geographically different.Therefore, it is important to develop a rapid and accurate methodology for clinical diagnosis. Current methods for detecting bacteria and bacterial components in cerebrospinal fluid, such as polymorphonuclear cells, Gram stain, latex agglutination(LA), acridine orange stain, and quellung capsular reaction, are not sensitive and specific enough. In addition, they are easily interfered with cross-reaction. The present study aimed to develop a highly bacteria species-specific and rapid method for the diagnosis of acute bacterial meningitis. First, we modified the DNA extraction procedure from QIAGEN commercial kit and established a mutiplex-PCR condition to target the DNA of different bacterial strains. Then, we evaluated the detection sensitivity of optimized mutiplex-PCR for these strains. Our preliminary results suggest that the common strains related to meningitis can be identified by using mutiplex-PCR.