| Summary: | 碩士 === 中國醫藥大學 === 醫學檢驗生物技術學系碩士班 === 98 === Galangin is a naturally flavonoid, which present in high concentrations in Alpina Officinarum Hance. Previous studies demonstrated that galangin has anti-inflammation, anti-oxidative properties and induces apoptosis in hepatoma and leukemia cell lines. Therefore, galangin may be a promising cancer chemopreventive agent. But it is unclear of mechanism to inhibit the growth of nasopharyngeal carcinoma cell lines. Our preliminary results show that galangin could induce apoptosis in human NPC cells, were characterized by nuclear condensation, DNA fragmentation and increased apoptotic sub-G1 population. Galangin inhibits cell growth by blocking cell cycle progression at S phase and the down-regulation of cyclin A and Cdk2 expression. Moreover, galangin treatment is strongly induced p53 expression and caspase-3 activation. The expression of endoplasmic reticulum (ER) stress sensors glucose-regulated protein 78 (GRP 78), PKR-like ER kinase (PERK), activating transcription factor 6 (ATF6), caspase-3 and -12 activities, and Ca++ release from endoplasmic reticulum were also induced after treatment with galangin. These results suggest that the ER stress and Ca++-dependent pathways may be involved in galangin-induced apoptosis in NPC cells. However, the molecular mechanism underlying the galangin-induced apoptotic process remains to be investigated further.
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