| Summary: | 碩士 === 中國醫藥大學 === 基礎醫學研究所 === 98 === The glioma is the most difficult to treat of primary tumor, has been difficult to
manage after chemicaltherapy, radiotherapy and surgical. Our major focus on our
laboratory is to develop and find a new anti-cancer drug for glioma treatment. Chrysin,
a flavonoid which was extracted from propolis, honey and plants, has been reported to
have anti-cancerous, anti-oxidative, and anti-inflammatory effects. Proplis is well
known in Taiwan as a traditional Chinese medicine. The results indicated that chrysin
induced morphological changes and cell death in human brain cancer GBM 8401 cells
and these effects are in time- and dose-dependent manners. We also found that chrysin
(Proplis extracts) caused G2/M phase arrest and apoptosis. Moreover, DAPI staining,
DNA electrophoresis, Comet assay, and annexin?nⅤ?nalso showed that chrysin induced
apoptosis. Furthermore, we suggest that chrysin promoted the levels of reactive
oxygen species (ROS), NO and Ca2+ concentration, Cardiolipin oxidation, but
decreased mitochondrial membrane potential in GBM 8401 cells. Interestingly, we are
focused on the protein levels and mRNA expressions, AIF and Endo G were
up-regulated but Caspase-3, Caspase-8 and Caspase-9 were changes a little. And we
demonstrate that chrysin induced cell death via caspase-independent mitochondrial
pathway which is involved in apoptosis from GBM 8401 cells after were incubated
for different time periods. In addition, by adding ROS or Calaium inhibitor, cell death
induced by chrysin could be significantly rose, but it could not be reversed by general
caspase inhibitor. In conclusion, chrysin has anti-brain cancer effects on GBM 8401
cells through apoptosis and cell cycle. Thus, chrysin can be a new anti-cancer drug
and used in clinical therapy.
|
|---|
