Chrysin Induces Cell Cycle Arrest and Apoptosis via Caspase-independent Pathway in GBM 8401 Cells.

• Main Authors: Shih-Hui Lin, 林詩慧
• Other Authors: 鍾景光
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/28249265884405180934

碩士 === 中國醫藥大學 === 基礎醫學研究所 === 98 === The glioma is the most difficult to treat of primary tumor, has been difficult to manage after chemicaltherapy, radiotherapy and surgical. Our major focus on our laboratory is to develop and find a new anti-cancer drug for glioma treatment. Chrysin, a flavonoid which was extracted from propolis, honey and plants, has been reported to have anti-cancerous, anti-oxidative, and anti-inflammatory effects. Proplis is well known in Taiwan as a traditional Chinese medicine. The results indicated that chrysin induced morphological changes and cell death in human brain cancer GBM 8401 cells and these effects are in time- and dose-dependent manners. We also found that chrysin (Proplis extracts) caused G2/M phase arrest and apoptosis. Moreover, DAPI staining, DNA electrophoresis, Comet assay, and annexin?nⅤ?nalso showed that chrysin induced apoptosis. Furthermore, we suggest that chrysin promoted the levels of reactive oxygen species (ROS), NO and Ca2+ concentration, Cardiolipin oxidation, but decreased mitochondrial membrane potential in GBM 8401 cells. Interestingly, we are focused on the protein levels and mRNA expressions, AIF and Endo G were up-regulated but Caspase-3, Caspase-8 and Caspase-9 were changes a little. And we demonstrate that chrysin induced cell death via caspase-independent mitochondrial pathway which is involved in apoptosis from GBM 8401 cells after were incubated for different time periods. In addition, by adding ROS or Calaium inhibitor, cell death induced by chrysin could be significantly rose, but it could not be reversed by general caspase inhibitor. In conclusion, chrysin has anti-brain cancer effects on GBM 8401 cells through apoptosis and cell cycle. Thus, chrysin can be a new anti-cancer drug and used in clinical therapy.