Development of in situ for method for monitoring monoamine neurotransmitter and metabolite levels in rat corpus striatum
碩士 === 嘉南藥理科技大學 === 藥物科技研究所 === 98 === This study combined microdialysis sampling with high performance liquid chromatography-electrochemical detection (HPLC-ECD) for the determination of the monoamine neurotransmitters and metabolite levels in the rat corpus striatum. The material of probe membrane...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2010
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| Online Access: | http://ndltd.ncl.edu.tw/handle/90801766143599816136 |
| Summary: | 碩士 === 嘉南藥理科技大學 === 藥物科技研究所 === 98 === This study combined microdialysis sampling with high performance liquid chromatography-electrochemical detection (HPLC-ECD) for the determination of the monoamine neurotransmitters and metabolite levels in the rat corpus striatum.
The material of probe membrane (length 4mm) was poly- arylethersulfone (PAES) and the cut-offs of these membranes were 20,000 Daltons. Separation of the monoamine neurotransmitters was obtained by a C18 ODS-3μm 100 mm × 4.6 mm column at a mobile phase consisting of 9% methanol in buffer (0.2mM sodium octyl sulfonate (SOS) and 0.05mM ethylene diamine tetraacetic acid disodium salt (EDTA Na2) with phosphoric acid to pH of 3), operated at a flow rate of 1ml/min and a inject volume of 20μl .A potential seep of +0.6V was applied across the working and reference electrodes of the detector.
A Simple high-performance liquid chromatography with electrochemical detection using microdialysis was developed for simultaneous determination of monoamine neurotransmitters and metabolite levels in the rat corpus striatum. The detection limits of these compounds were 0.3~127nM, the correlation coefficients of regression were more than 0.99.
The method has been successfully applied to measure the neurotransmitters and their metabolite levels in the rat corpus striatum by morphine injection in acute and chronic morphine treatment.
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