Analyzing the Expression of Protein Kinase C Family in Human Hepatocellular Carcinoma

• Main Authors: Hsiu-Chin, 盧秀琴
• Other Authors: Fen-Pi Chou
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/28771230713112664913

博士 === 中山醫學大學 === 生化暨生物科技研究所 === 98 === Protein kinase Cs(PKCs) play important roles in cell proliferation, differentiation, apoptosis, migration and tumorigenesis. In the present study, we analyzed the expression of PKCs in human hepatocellular carcinoma (HCC) tissues and explored their roles in the development of HCC., we used real-time quantitative RT-PCR, mutation analysis, and immunohistochemical staining to investigate the expression of PKCs in 50 pairs of human hepatocellular carcinoma (HCC) tissues. Results showed that PKC β and PKCθ were down-regulated in HCC tissues. Reduced expression of PKC θ is well correlated with the grade of cancer cells (p=0.009), and the down-regulated expression of PKC βII is associated with HBV infection (P=0.035). From these results, we suggest that the expression of PKC θ and PKC β may have clinical significance in HCC. Specialy, our data showed that the expression of PKCη is down-regulated in 82% of HCC tissues. The down-regulation is associated with poorer long-term survival of HCC patients. These results suggest that PKC η plays a tumor suppressor-like role in the development of HCC.