Celebrex Decelerate Ischemia-Reperfusion Caused Skeletal Muscle Damage

• Main Authors: Chien-Sheng, 羅健生
• Other Authors: 王朝鐘
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/19586527305703345026

碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 98 === It is necessary to use tourniquet for reducing blood flow under surgical progression although it can cause Ischemia/Reperfusion (I/R) damage to induce inflammation and swelling after surgery. Celebrex is a well-known selective Cyclooxygenase-2 (COX-2) inhibitor used in clinic to treat inflammation. In this study, we aimed to use limb ischemic model in rat to mimic a tourniquet-caused ischemic condition in skeletal muscle, and we tried to observe the ability of celebrex in inhibiting I/R casued inflammation. The results showed that celebrex can reduce I/R caused increase of IL-1β.It was also observed that I/R caused COX-2 protein was reduced as pretreating with celebrex accompanying by the regulation of P-JNK, P-P38 and PI3K. Taken together, we found celebrex possess the ability to reduce I/R caused inflammation in skeletal muscle.