Study of the Efficiency of Biological Hydrogen Generation by FCY1 Strain - Effects of Metallic Ions and Suspended Solids

• Main Authors: Chia-Hao Kuo, 郭家豪
• Other Authors: Sung-Nung Lee
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/08485064912222928621

碩士 === 輔仁大學 === 化學系 === 98 === A newly isolated H2-production strain, FCY1, was studied for its capacity of fermentative H2 production in a continuous-flow stirred tank reactor (CSTR). When glucose was used as carbon source, supplemented with metallic ions and suspended solids to investigate the hydrogen production efficiency. In this study,the hydrogen-generating capability of the bacterium strain FCY1,sorted from Yang-Ming-Shang hot spring has been confirmed by our lab. The strain FCY1 is accredited judge for the Gram-negative bacilli (Acinetobacter calcoaceticus) and current literature in the hydrogen production using anaerobic microorganisms is different. In addition,adding the metallic ions to the medium has different effects in hydrogen production. The condition is Al 2 (SO4 )3 ＞ CaSO4 = MgSO4 ＞ FeSO4 > MnSo4 > CuSO4 > NiSO4 > Na2 SO4. Only metallic ions for hydrogen production, the condition for hydrogen concentration is Fe +2 > Ca +2 > Mg + > Mn +2 > Na + > Al +3 > Ni +2 > Cu +2. Therefore,iron ion, calciumn ion and magnesium ion at 0.001 mole/L exhibit the better efficiency in hydrogen generation. On the other hand, in order to have better surface area to microbial growth and attachment, and also improve the overall biomass in the reactor by adding porous material. Biohydrogen production in CSTR with different support matrices,(such as Molekularsieb, Biochemical filter sets, Activated carbon, Multiples bio-ceramic ring, Polyvinyl alcohol) were examined. It was found the bioreactor with activated carbon(when HRT=4 hr) has the best efficiency. The maximal H2 yield and H2 production rate were estimated to be 10.82 mol- H2/ mole- glucose and 0.633 L/h/L respectively. (H2 concentration 58% )