Effects of chronic riboflavin deficiency on the biochemical indicators and gene expression of rat liver
碩士 === 輔仁大學 === 營養科學系 === 98 === Marginal and deficient riboflavin status has been prevalent in Taiwan. In eukaryotic cells, riboflavin participates in a range of metabolic redox reactions in its coenzyme forms. The objectives of our study were 1) to establish the chronic B2 deficiency animal model,...
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ndltd-TW-098FJU005130032015-11-13T04:09:15Z http://ndltd.ncl.edu.tw/handle/34448565807198884498 Effects of chronic riboflavin deficiency on the biochemical indicators and gene expression of rat liver 慢性維生素B2缺乏過程對大白鼠維生素B2營養生化指標與肝臟基因表現之影響 Yi-Ting Shih 施懿庭 碩士 輔仁大學 營養科學系 98 Marginal and deficient riboflavin status has been prevalent in Taiwan. In eukaryotic cells, riboflavin participates in a range of metabolic redox reactions in its coenzyme forms. The objectives of our study were 1) to establish the chronic B2 deficiency animal model, 2) to measure the effects of B2 deficiency on B2-dependent biochemical indicators, 3) to investigate the changes in gene expression due to B2 status by microarray analysis. Sixty 3-weeks-old weanling male Sprague-Dawley rats were allocated to one of the four treatment regiments, being fed either a riboflavin-adequate diet (control) ad lib, riboflavin adequate diet (pair-fed), riboflavin-deficiency diet ad lib, or riboflavin deficiency with 20 % (wt/wt) fat diet (ad lib) for 3, 7 and 12 weeks to establish the chronic riboflavin-deficient animal models. Riboflavin status was checked by erythrocyte glutathione reductase activation coefficient (EGRAC) every other week. Food intake, growth rate and the pathological signs were proportional to riboflavin content. Lower levels of organ riboflavin contents and higer EGRAC were observed in B2 deficiency and high fat groups. The activites of heart glutathione reuctase, hepatic and heart pyridoxamine (pyridoxine) 5’-phosphate oxidase, hepatic and renal succinate dehydrogenase were significantly lower in riboflavin-deficient groups than in riboflavin-adequate groups. Moreover, riboflavin deficiency alone was associated with changes in expression of clusters of genes that play roles in energy metabolism, nutrients metabolism, cell cycle progression and detoxification systems. Riboflavin deficiency with calorie-restriction was associated with changes in expression of clusters of genes involved in energy metabolism, detoxification systems, cell structures and immune systems. Riboflavin deficiency with high fat diet was associated with changes in expression of clusters of genes in energy metabolism, apoptosis, nervous systems and oxidative stress. Combination of riboflavin deficiency, calorie-restriction and high fat affected the genes expression of energy metabolism, cell cycle progression, nervous systems, detoxification systems and aging. We concluded that riboflavin deficiency resulted in changes in enzyme activities and a widespread gene expression of rat livers. Feili Lo Yang 駱菲莉 學位論文 ; thesis 169 zh-TW |
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碩士 === 輔仁大學 === 營養科學系 === 98 === Marginal and deficient riboflavin status has been prevalent in Taiwan. In eukaryotic cells, riboflavin participates in a range of metabolic redox reactions in its coenzyme forms. The objectives of our study were 1) to establish the chronic B2 deficiency animal model, 2) to measure the effects of B2 deficiency on B2-dependent biochemical indicators, 3) to investigate the changes in gene expression due to B2 status by microarray analysis. Sixty 3-weeks-old weanling male Sprague-Dawley rats were allocated to one of the four treatment regiments, being fed either a riboflavin-adequate diet (control) ad lib, riboflavin adequate diet (pair-fed), riboflavin-deficiency diet ad lib, or riboflavin deficiency with 20 % (wt/wt) fat diet (ad lib) for 3, 7 and 12 weeks to establish the chronic riboflavin-deficient animal models. Riboflavin status was checked by erythrocyte glutathione reductase activation coefficient (EGRAC) every other week. Food intake, growth rate and the pathological signs were proportional to riboflavin content. Lower levels of organ riboflavin contents and higer EGRAC were observed in B2 deficiency and high fat groups. The activites of heart glutathione reuctase, hepatic and heart pyridoxamine (pyridoxine) 5’-phosphate oxidase, hepatic and renal succinate dehydrogenase were significantly lower in riboflavin-deficient groups than in riboflavin-adequate groups. Moreover, riboflavin deficiency alone was associated with changes in expression of clusters of genes that play roles in energy metabolism, nutrients metabolism, cell cycle progression and detoxification systems. Riboflavin deficiency with calorie-restriction was associated with changes in expression of clusters of genes involved in energy metabolism, detoxification systems, cell structures and immune systems. Riboflavin deficiency with high fat diet was associated with changes in expression of clusters of genes in energy metabolism, apoptosis, nervous systems and oxidative stress. Combination of riboflavin deficiency, calorie-restriction and high fat affected the genes expression of energy metabolism, cell cycle progression, nervous systems, detoxification systems and aging. We concluded that riboflavin deficiency resulted in changes in enzyme activities and a widespread gene expression of rat livers.
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author2 |
Feili Lo Yang |
author_facet |
Feili Lo Yang Yi-Ting Shih 施懿庭 |
author |
Yi-Ting Shih 施懿庭 |
spellingShingle |
Yi-Ting Shih 施懿庭 Effects of chronic riboflavin deficiency on the biochemical indicators and gene expression of rat liver |
author_sort |
Yi-Ting Shih |
title |
Effects of chronic riboflavin deficiency on the biochemical indicators and gene expression of rat liver |
title_short |
Effects of chronic riboflavin deficiency on the biochemical indicators and gene expression of rat liver |
title_full |
Effects of chronic riboflavin deficiency on the biochemical indicators and gene expression of rat liver |
title_fullStr |
Effects of chronic riboflavin deficiency on the biochemical indicators and gene expression of rat liver |
title_full_unstemmed |
Effects of chronic riboflavin deficiency on the biochemical indicators and gene expression of rat liver |
title_sort |
effects of chronic riboflavin deficiency on the biochemical indicators and gene expression of rat liver |
url |
http://ndltd.ncl.edu.tw/handle/34448565807198884498 |
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