| Summary: | 碩士 === 弘光科技大學 === 生物科技研究所 === 98 === Paederia foetida is cultivated in warm regions of the world as an ornamental vine and a variety of curative properties have been attributed to the plant in folk medicine. It has been documented that Paederia foetida had the effects on anti-tumor, anti-tussive, anti-diarrheal and anti- bacteria.
Macrophages play a vital role in host defense against noxious substances and are involved in a variety of disease process, including autoimmune disease, infections and inflammatory disorders. Hence, we intended to imitate the physiologically infectious and inflammatory process
by stimulating RAW264.7 macrophage cell line with lipopolysaccharide and evaluate the effect of Paederia foetida extract on the production of inflammatory mediators and reveal its regulatory mechanisms.
The yield of Paederia foetida extract was 25.3% and the contents of total polyphenol compounds and flavonoids were 241.1 mg /100g D.W and 205.4 mg /100g D.W, respectively. The results of this study showed PFE could significantly inhibit the productions of NO, TNF-a and IL-6 in
LPS-stimulated RAW264.7 cells (P < 0.05). Furthermore, PFE could effectively inhibit the degradation of I-kB , decrease the activation of NF-kB and reduce the formation of iNOS formation. In condusion, it suggested that the anti-inflammatory activity of PFE due to the down-regulation of IL-6, NO, and TNF-a via the suppression of NF-kB
activation.These findings implicate that Paederia foetida applied to the regulation of macrophage-mediated inflammatory diseases and may be served as the alternative medicine for ameliorating inflammatory abnormatiy.
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