The Interaction of the Human Cytomegalovirus UL76 with S5a

• Main Authors: Cheng-Hui Hu, 胡臣蕙
• Other Authors: Shang-Kwei Wang
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/44221293688762687192

碩士 === 高雄醫學大學 === 醫學研究所 === 98 === Human cytomegalovirus (HCMV) UL76 protein belongs to a highly conserved protein family UL24 of herpesviruses. UL76 is known to induce chromosomes aberrations. Previously, Dr. Wang’s group has shown that UL76 interacts with S5a, a subunit of the ubiquitin-proteasome system (UPS) in a yeast two-hybrid CytoTrap screen. In order to further investigate the interaction of UL76 and S5a, we performed multiple protein-protein interaction assays. Here we demonstrated that the UL76 interacts with full-length S5a by the yeast two-hybrid system. Furthermore, immunofluorescence cell staining showed that UL76 appears to localize with S5a in the nucleus. From co-immunoprecipitation assay, we showed that UL76 interacts with S5a. Taken together, we propose that UL76 interacts with S5a might represent a novel strategy to modulate the HCMV-infected cells.