| Summary: | 碩士 === 高雄醫學大學 === 藥學研究所 === 98 === This study describes approaches for stacking and analyzing of androstenedione, testosterone, epitestosterone, boldenone and clostebol in urine by sweeping capillary electrophoresis. After liquid-liquid extraction with n-hexane, the extracted samples were loaded by hydrodynamic injection (10 psi, 99.9 s). The stacking and separation were performed at -20 kV and detected at 254 nm using phosphate buffer (30 mM, pH 5.0) containing 100 mM sodium dodecyl sulfate and 40% methanol. During method validation, calibration curves were linear (r ≥ 0.990) over a range 50 - 1000 ng/mL. For the evaluation of precision and accuracy, the RSD and RE were all less than 6.6% in intra- and inter-day analysis. The limits of detection were 30 ng/mL (S/N = 5, sampling 99.9 s at 10 psi). This study could be applied for doping monitoring.
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