Interaction Analysis between Microtubule Actin Crosslinking Factor 1 (MACF1) and Desmoplakin
碩士 === 銘傳大學 === 生物科技學系碩士班 === 98 === According to the previous studies, Microtubule actin crosslinking factor 1 b (MACF1b) could co-localize with desmoplakin (DP) by immuno-fluorescent staining. However, whether MACF1b really interacts with DP remains to be determined. The main purpose of my thesis...
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ndltd-TW-098MCU051110072015-10-13T19:06:46Z http://ndltd.ncl.edu.tw/handle/35777771502095135932 Interaction Analysis between Microtubule Actin Crosslinking Factor 1 (MACF1) and Desmoplakin Microtubule Actin Crosslinking Factor 1 (MACF1)和Desmoplakin之間交互作用的分析 Chih-Ying Hsu 許志盈 碩士 銘傳大學 生物科技學系碩士班 98 According to the previous studies, Microtubule actin crosslinking factor 1 b (MACF1b) could co-localize with desmoplakin (DP) by immuno-fluorescent staining. However, whether MACF1b really interacts with DP remains to be determined. The main purpose of my thesis is to study their direct interactions between MACF1b and DP. Preliminary data shows that MACF1b can be immuno-precipitated by DP, and MACF1b PRDs (plakin repeat domains) interacts with desmoplakin C terminus. We have divided desmoplakin C terminus into three fragments (desmoplakin-C1、desmoplakin-C2 desmoplakin-C3). According to the results of western blotting and co-imuno-precipitation of desmoplakin C fragments with MACF1b PRDs shows that DP-C2 and DP-C3 fragments could interact with MACF1b. Chung-Ming Lin 林重銘 2010 學位論文 ; thesis 70 zh-TW |
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碩士 === 銘傳大學 === 生物科技學系碩士班 === 98 === According to the previous studies, Microtubule actin crosslinking factor 1 b (MACF1b) could co-localize with desmoplakin (DP) by immuno-fluorescent staining. However, whether MACF1b really interacts with DP remains to be determined. The main purpose of my thesis is to study their direct interactions between MACF1b and DP. Preliminary data shows that MACF1b can be immuno-precipitated by DP, and MACF1b PRDs (plakin repeat domains) interacts with desmoplakin C terminus. We have divided desmoplakin C terminus into three fragments (desmoplakin-C1、desmoplakin-C2 desmoplakin-C3). According to the results of western blotting and co-imuno-precipitation of desmoplakin C fragments with MACF1b PRDs shows that DP-C2 and DP-C3 fragments could interact with MACF1b.
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author2 |
Chung-Ming Lin |
author_facet |
Chung-Ming Lin Chih-Ying Hsu 許志盈 |
author |
Chih-Ying Hsu 許志盈 |
spellingShingle |
Chih-Ying Hsu 許志盈 Interaction Analysis between Microtubule Actin Crosslinking Factor 1 (MACF1) and Desmoplakin |
author_sort |
Chih-Ying Hsu |
title |
Interaction Analysis between Microtubule Actin Crosslinking Factor 1 (MACF1) and Desmoplakin |
title_short |
Interaction Analysis between Microtubule Actin Crosslinking Factor 1 (MACF1) and Desmoplakin |
title_full |
Interaction Analysis between Microtubule Actin Crosslinking Factor 1 (MACF1) and Desmoplakin |
title_fullStr |
Interaction Analysis between Microtubule Actin Crosslinking Factor 1 (MACF1) and Desmoplakin |
title_full_unstemmed |
Interaction Analysis between Microtubule Actin Crosslinking Factor 1 (MACF1) and Desmoplakin |
title_sort |
interaction analysis between microtubule actin crosslinking factor 1 (macf1) and desmoplakin |
publishDate |
2010 |
url |
http://ndltd.ncl.edu.tw/handle/35777771502095135932 |
work_keys_str_mv |
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