Interaction Analysis between Microtubule Actin Crosslinking Factor 1 (MACF1) and Desmoplakin

• Main Authors: Chih-Ying Hsu, 許志盈
• Other Authors: Chung-Ming Lin
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/35777771502095135932

碩士 === 銘傳大學 === 生物科技學系碩士班 === 98 === According to the previous studies, Microtubule actin crosslinking factor 1 b (MACF1b) could co-localize with desmoplakin (DP) by immuno-fluorescent staining. However, whether MACF1b really interacts with DP remains to be determined. The main purpose of my thesis is to study their direct interactions between MACF1b and DP. Preliminary data shows that MACF1b can be immuno-precipitated by DP, and MACF1b PRDs (plakin repeat domains) interacts with desmoplakin C terminus. We have divided desmoplakin C terminus into three fragments (desmoplakin-C1、desmoplakin-C2 desmoplakin-C3). According to the results of western blotting and co-imuno-precipitation of desmoplakin C fragments with MACF1b PRDs shows that DP-C2 and DP-C3 fragments could interact with MACF1b.