| Summary: | 碩士 === 美和技術學院 === 健康與生技產業研究所 === 98 === The chemical constituents of the Formosan soft corals Sinularia triangular were studied. This study led to the isolation of eight natural products (1–8), including one new quinine type metabolite (8), along with seven known compounds, sinularin (1), sinulariolide (2), 11-epi-sinulariolide acetate (3), querciformolide C (4), sinulariolone (5), 11-dehydrosinulariolide (6) and (Z)-N-[2-(4-hydroxyphenyl)ethyl]-3-methyldodec-2-enamide (7) from the soft coral S. triangular. The structures of 1–8 were elucidated by spectroscopic evidences (1D NMR, 2D NMR, IR, and MS) and by the comparison of those of related metabolites in literatures. The cytotoxicity of 1–8 against four human cancer cell lines, Hela (cervical epitheloid carcinoma), Hep 2 (laryngeal carcinoma), Daoy (medulloblastoma) and MCF-7 (breast carcinoma), was assayed. The result showed that eight metabolites (1–8) were inactive (ED50’s >20 μg/mL) towards these cancer cell lines. Furthermore, the in vitro anti-inflammatory effect of compounds 1–8 was tested. In this assay, compounds 1–3, 6, and 8 significantly inhibited the accumulation of the pro-inflammatory iNOS protein of the LPS-stimulated RAW264.7 macrophage cells at 10 μM. At the same concentrations, compound 3 also could significantly inhibit the accumulation of the pro-inflammatory COX-2 protein.
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