The isolation and characteristics study of environmental toxicant 2,3,7,8-TCDD biotransformation bacteria.

• Main Authors: Chen yu-chi, 陳郁綺
• Other Authors: Liou chao-lin
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/74205967114171341596

碩士 === 明志科技大學 === 生化工程研究所 === 98 === This study is to dioxin contaminated soil from the local to find out the dioxin biotransformation bacteria and bacteria through the analysis of the biochemical characteristics of the test, reaching biotransformation bacteria, isolation and acclimation purposes. This study collected from contaminated sites in the three soil samples , analyze results of dioxin pollution, dioxin toxic equivalent concentration of up to 2450 ng-TEQ / kg, the concentration of control over the soil standard. Four bacteria strains which can survive in dioxin media were isolated after sub- culture inoculation. The four strains were identified as Achromobacter xylosoxidans, Ochrobactrum anthropi, Ralstonia mannitolilytica and Agromyces sp. . Achromobacter xylosoxidans, Ochrobactrum anthropi, Agromyces sp. could degrade 2,3,7,8-TCDD at lag phase, whereas Ralstonia mannitolilytica decomposed it primarily at log phase. It was also observed that Agromyces sp. had the maximum 2,3,7,8-TCDD degradation efficiency (97%). In addition, proteomics through to observe the Agromyces sp. and Achromobacter xylosoxidans proteins that change, and through the MALDI-TOF MS to understand its role.