The isolation and characteristics study of cellulose-decomposition bacteria.

• Main Authors: Chang han-chieh, 張瀚介
• Other Authors: Chen hsi-chin
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/83451141568603458160

碩士 === 明志科技大學 === 生化工程研究所 === 99 === The objective of this study was to isolate the indigenous cellulose-degrading bacteria of Taiwan. Also, the characteristics of these bacteria were investigated after isolation followed by culture enrichment and strains acclimation. In this study, Three soil samples were gathered from hot spring soil, Yangmingshan soil and gas station soil, removed from the strain of bacteria sources through ten subcultures and separation, can be isolated and identified five cellulose as the sole carbon source to survive in the medium. In this study, 16SrDNA DNA sequence of strain identification system for strain identification, analysis showed that five decomposing bacteria were Sphingomonas sp., Pseudomonas sp.M1, Achromobacter sp., Pseudomonas sp. M2,Stenotrophomonas sp..Use Gram staining and optical electron microscope in 1000 multiple found strains, in this study, it was found that all of the strains are gram’s negative in the morphology, and the exterior for the bacteria, five strains of bacteria in the Pseudomonas sp.M1 length of about 3μm and width of 1μm, Pseudomonas sp.M2 and Stenotrophomonas sp. length of about 2μm and width of 1μm, Sphingomonas sp. and Achromobacter sp. length and width of about 1μm is the smallest, while the capacity for cellulose degradation by bacteria of the maximum of Pseudomonas sp. M1 is the best, by the growth kinetics of microbial cellulose calculate the dependence of the strain without a high degree of inhibition of the case, the maximum specific growth rate μmax to 0.439hr-1, the half-saturation constant Ks is 776 mg/L,it is also the highest yield of reducing sugar, per gram of CMC can produce 51.36 mg of reducing sugar. In addition, also can be confirmed by polymerase chain reaction(PCR) by the decomposition of selected bacteria with the cellulose-degrading gene fragment of decomposing bacteria, so the strains screened in this study are all indigenous cellulose-degrading bacteria of Taiwan.