Immobilization of Candida rugosa Lipase on Polyvinylidene Fluoride Membrane

• Main Authors: Cheng-Chung Yu, 游承璋
• Other Authors: 劉永銓
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/58064236997781516282

碩士 === 國立中興大學 === 化學工程學系所 === 98 === The Polyvinylidene Fluoride Membrane (PVDF) was used as solid matrix for lipase immobilization. The modification was conducted by coupling 1,4-diaminobutane (DA) as the spacer, followed by binding the active reagents (i.e., GA, EPI, TCT) on the surface of PVDF. Study on four immobilization method (i.e., Native PVDF, GA, EPI, TCT), Use EPI-modified PVDF showed the best effect on lipase immobilization. When using 1.5M Sodium hydroxide (NaOH) for coupling Epichlorohydrin (EPI) on PVDF, the membrane surface was observed to form many nanoparticles. It is expected that the nanoparticle provides huge specific surface areas for lipase to be immobilized. In comparison of the kinetic parameter, Vmax, of the immobilizaed lipase is similar to that of the free lipase. The activity yield of the immobilized lipase was 97.1% of the free enzyme under the optimal immobilization conditions. The EPI-modified PVDF displayed a dramatic improvement in lipase performance in all aspects. It showed a 92% residual activity for 30-days storage and a 88% activity reservation for a 15-times repeated use.