Investigation on cellulase immobilization with Polyvinyl Alcohol

• Main Authors: Rong -Cih Chang, 張蓉慈
• Other Authors: 劉永銓
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/35915527643270511726

碩士 === 國立中興大學 === 化學工程學系所 === 98 === In this study,various matrix inclubing regenerated cellulose membrane(RCM),glass fiber membrane(GFM), polyvinyl alcohol membrane(PVAM), poly vinylidene fluoride membrane (PVDFM)、polyether sulfone membrane (PESM) were used for cellulase immobilization.Glutaraldehyde was used as the crosslinking reagent coupling with the amine of cellulase.Among them,RCM gave the highest cellulase activity,whereas,it will lose its activity rapidly due to the digestion of cellulase on the RCM.Both PVDFM and PESM are improper matrix for cellulase immobilization due to the hydrophobic characteristics.Though GFM is capable of coupling cellulase,its structure is rather agile in aqueous solution. Polyvinyl alcohol as the matrix for cellulase immobilization can endure more than 3 times repeated use was chosen as the matrix for further study. In this study, it is found that polyvinyl alcohol along with glutaraldehyde as the covalent reagent to simultaneously entrap and bind cellulase gave the best result for cellulase immobilization.When using 6% glutaraldehyde as the reagent,the PVA membrane was structurally stable in aqueous solution.In the stability test, the PVA-immobilized cellulase can undergo more than 10 times repeated use with 18.45% residual activity retained.In the storage test,the polyvinyl alcohol membrane immersed in pH 7 phosphate buffer can maintained 52.95% of its activity after 4 –day storage.