Expression of mitofusion-2 in non-small cell lung cancer

• Main Authors: Hung-Chen Liao, 廖紅禎
• Other Authors: 周寬基
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/99962672208367871017

碩士 === 國立中興大學 === 生命科學院碩士在職專班 === 98 === Mitochondria are key players in several cellular functions including growth, division,energy metabolism, and apoptosis. Mitochondrial dysfunction contributes to a number of human disorders and may aid cancer progression. Although normal mitochondrial morphology is important for the function and physiology of cells, defects in mitochondrial dynamics may cause severe diseases. Fusion and fission of mitochondria regulate their morphology and distribution. Mitofusin-2（Mfn-2）is an outer mitochondrial membrane protein. Recent observations indicate that Mfn2 is a multifunctional membrane protein that participates in cell proliferation and metabolism and that it is required for normal endoplasmic reticulum morphology. The roles of Mfn-2 in cancers are largely unknown. In this study, which aims at defining the role of Mfn-2 in lung cancer, we analyzed the expression of Mfn-2 in non-small cell lung cancer（NSCLS） using immunohistochemistry . Mfn-2 expression that was detected in the cytoplasm in average immunostaining of Mfn2 was 80％ for tumor and 20％ for non-tumor（Pearson Chi-Square；p＜0.05）.We analyzed the expression of Mfn-2 in tumor and non-tumor pairs by using western blot. Increased expression of Mfn-2 in malignant cells compared to the non-tumor cells suggests that Mfn-2 expression may play a role in NSCLS aggravation. It may be a novel marker for diagnosis and treatment of NSCLS.