| Summary: | 碩士 === 國立中興大學 === 微生物暨公共衛生學研究所 === 98 === In poultry, recombinant fowlpox virus (rFPV) - vectored vaccine is a useful method to prevent the loss of productivity due to fatal infectious diseases. To increase the host immune responses, vaccine combining with immunostimulatory factors as adjuvants is a very significant point. For instance, as a powerful immunoregulatory cytokine, chicken interferon-γ (chIFN-γ) involves in innate and adaptive immunity and plays an important role to defense infectious diseases. Similar to human, the secretion of chIFN-γcould be also induced strongly by chicken interleukin (chIL)-12 and chIL-18 costimulation. ChIL18-encoding region has been inserted into F11L gene of FPV DNA to generate recombinant virus, rFPV-chIL18. Investigation of the relationship between the expression of chIL-18 and chIFN-γproduction in chickens vaccined with rFPV is the key point to determine if rFPV-chIL18 can enhance chicken immunity. In this experiment, two groups of chickens given rFPV-chIL18 (1×106.48TCID50) by wing-web puncture at one-week-old and three-week-old, respectively. Using quantitative polymerase chain reaction to detect chIL18 mRNA expression in pox-like lesions (nodules) and enzyme-linked immunosorbent assay to detect chIFN-γlevels in sera, nodules and splenocyte culture supernatants. In nodules, high level of chIL18 mRNA expression and chIFN-γ production was detected at the first three days after postinoculation (dpi), another high level chIFN-γ at 7 dpi. The highest level of chIFN-γwas detected at 5 dpi in sera. In the splenocyte culture supernatants, the highest level of chIFN-γwas detected at 7 dpi to 14 dpi. The results not only show that chIL-18 could really enhance chIFN-γproduction but also indicate that systemic chIFN-γproduction may use different mechanisms.
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