Characterization of microsatellite DNA and genetic structure of diploid Pteris faurieri in Taiwan

碩士 === 國立成功大學 === 生物多樣性研究所 === 98 === There are two varieties of Pteris fauriei in Taiwan, i.e. fauriei and minor. P. fauriei var. minor is diploid and P. fauriei var. faurie is triploid. In general, diploid plants occur in warmer habitats, while triploid plants grow at grassland and woodland. P. fa...

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Bibliographic Details
Main Authors: Chu-ChunChien, 簡竹君
Other Authors: Tzen-Yuh Chiang
Format: Others
Language:zh-TW
Published: 2010
Online Access:http://ndltd.ncl.edu.tw/handle/97470312575988716807
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Summary:碩士 === 國立成功大學 === 生物多樣性研究所 === 98 === There are two varieties of Pteris fauriei in Taiwan, i.e. fauriei and minor. P. fauriei var. minor is diploid and P. fauriei var. faurie is triploid. In general, diploid plants occur in warmer habitats, while triploid plants grow at grassland and woodland. P. fauriei var. minor is widespread in Taiwan and nearby islets, except for central Taiwan and Matsu islands. In contrast, P. fauriei var. fauri is not found in southern Taiwan or islets in the Taiwan Strait. This study focuses on the population genetics of P. fauriei var. minor and the origin of P. fauriei var. faurie. In this study, 11 microsatellite loci were isolated from P. fauriei var. minor for population genetic analysis and can also be amplified with P. fauriei var. faurie. Four were cross-amplified with 21 Pteris species. The average of gene diversity is high (0.772), the number of allelic richness from 4.071 to 12.221, and rare allele number is also high (1 to 9). AMOVA indicates that most genetic variation resides within populations (91.75%), resulting in low genetic differentiation (FST= 0.083). Lack of geographical subdivision was further supported by STRUCTURE analysis. In conclusion, all loci can be amplified with P. fauriei var. fauriei, suggesting its autopolyploidy. Low population differentiation is likely due to frequent gene flow between P. fauriei var. minor populations.