The effect of hyaluronan on endothelial differentiation of placenta-derived mesenchymal stem cells

• Main Authors: Chia-JungCheng, 鄭家蓉
• Other Authors: Lynn L.H. Huang
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/87303331072994119571

碩士 === 國立成功大學 === 生物科技研究所碩博士班 === 98 === During wound healing, angiogenesis plays a key role in regeneration of new blood vessels for delivery of oxygen and nutrients to repairing site. How to choose an excellent cell source and components of microenvironment for angiogenesis is important in tissue engineering. We investigated the endothelial differentiation of placenta-derived mesenchymal stem cells (PDMSCs), a population of endothelial marker KDR/VE-cadherin/CD31/vWF-negative cells. PDMSCs, cultured in EGM-2 medium containing angiogenic growth factors or basal medium DMEM-10% FBS for 21 days, showed high expressions both in gene and protein levels of endothelial markers. The time course of endothelial differentiation from day 7 to day 32 for PDMSCs showed increased levels of mRNA encoding KDR, VE-cadherin, CD31, vWF and VEGF at day 14 or day 18, and reached the highest level at day 21. P7 expressed high level of mRNA earlier than P5 PDMSCs, indicating P7 PDMSCs had more potential of differentiation into endothelial cells (ECs). Addition of low molecular weight (4300 Da) or high molecular weight (1500 kDa) hyaluronan (HA) with concentration 10 μg/mL, 100 μg/mL and 1000 μg/mL to DMEM-10% FBS medium showed that expression of some endothelial markers were enhanced by 4300 Da 10 μg/mL and 1500 kDa 10/100/1000 μg/mL HA. In conclusion, these results suggest that PDMSCs cultured in DMEM-10% FBS medium for 21 days can be induced into endothelial differentiation, and HA promotes gene and protein expression of endothelial markers, demonstrating HA has angiogenic potential.