Gene Expression in Caloric Restriction and Pathway Analysis

• Main Authors: Yi-JingTzseng, 曾怡靜
• Other Authors: Shih-Huang Chan
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/70696733042031315377

碩士 === 國立成功大學 === 統計學系碩博士班 === 98 === Most of the biological phenomena or disease is the result of the interactions of multiple genes. Extensive studies on the restriction of caloric (CR) intake showed that CR can extend the longevity in many organisms. Wang (2009) examined the expression level of 210 T.vaginals protein kinase genes under CR by using time-course cDNA microarray. In this thesis, we smoothed the gene expression of the 210 protein kinase, then used piecewise linear model to estimate the changing time that gene expression is dramatically different from the previous behavior, and the rate of gene expression. Applying complete linkage clustering method, thirty-two genes are found to have large changing point or with increasing pattern in gene expression after changing point. Statistical analysis shows that those patterns are highly significant, and four out of these thirty-two genes, TVAG_089630, TVAG_339340,TVAG_123500 and TVAG_456650，have been verified (Wang, 2010). The association of the found 32 genes is to be studied further, hoping that the interaction behaviors of these significant genes can be understood.